Possible involvement of maize Rop1 in the defence responses of plants to viral infection

被引：34
作者：
Cao, Yanyong [1 ,2 ,3 ]
Shi, Yan [1 ,2 ]
Li, Yongqiang [1 ,2 ]
Cheng, Yuqin [1 ,2 ]
Zhou, Tao [1 ,2 ]
Fan, Zaifeng [1 ,2 ]
机构：
[1] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
[2] China Agr Univ, Dept Plant Pathol, Beijing 100193, Peoples R China
[3] Henan Acad Agr Sci, Cereal Crops Inst, Zhengzhou 450002, Peoples R China
来源：
MOLECULAR PLANT PATHOLOGY | 2012年 / 13卷 / 07期
关键词：
MOSAIC-VIRUS; DOWN-REGULATION; EXPRESSION; GTPASE; RAC; PROTEIN; RNA; RESISTANCE; SEQUENCE; TOBACCO;
D O I：
10.1111/j.1364-3703.2011.00782.x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The expression of host genes can be altered during the process of viral infection. To investigate the viral infection-induced up-regulated gene expression changes of maize at different time intervals post-inoculation with Sugarcane mosaic virus (SCMV), a suppression subtractive hybridization cDNA library was constructed. A total of 454 cDNA clones were identified to be viral infection-induced up-regulated genes. The influence of Rop1 on the infection of maize by SCMV was investigated. The results showed that transient silencing of the ZmRop1 gene through virus-induced gene silencing enhanced the accumulation and systemic infection of SCMV and another potyvirus (Pennisetum mosaic virus) in maize plants, whereas transient over-expression of ZmRop1 in maize protoplasts reduced SCMV accumulation. Furthermore, it was demonstrated that the heterologous expression of ZmRop1 impaired Potato virus X infection in Nicotiana benthamiana plants. These data suggest that ZmRop1 may play a role in plant defence responses to viral infection.
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收藏
页码：732 / 743
页数：12
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