Isolation and Properties of Succinate Dehydrogenase Isozymes from Maize Scutellum (Zea mays L.)

被引：1
作者：
Eprintsev, A. T. [1 ]
Fedorin, D. N. [1 ]
Karabutova, L. A. [1 ]
Florez, O. [1 ]
Puglla, M. [1 ]
机构：
[1] Voronezh State Univ, Voronezh 394018, Russia
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2018年 / 54卷 / 01期
基金：
俄罗斯科学基金会;
关键词：
succinate dehydrogenase; isozymes; ion exchange chromatography; electrophoresis; maize; ARABIDOPSIS-THALIANA; COMPLEX-II; GENES; EXPRESSION; PROTEINS;
D O I：
10.1134/S0003683818010039
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Four electrophoretically homogeneous succinate dehydrogenase isoenzymes with specific activities of 0.041, 0.110, 0.030 and 0.037 U/mg protein were isolated and purified from maize scutellum (Zea mays L.). Their catalytic properties and pH optimums were studied.
引用
收藏
页码：34 / 37
页数：4
相关论文
共 22 条
  • [1] New insights into the regulation of plant succinate dehydrogenase - On the role of the protonmotive force
    Affourtit, C
    Krab, K
    Leach, GR
    Whitehouse, DG
    Moore, AL
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (35) : 32567 - 32574
  • [2] Ischaemic accumulation of succinate controls reperfusion injury through mitochondrial ROS
    Chouchani, Edward T.
    Pell, Victoria R.
    Gaude, Edoardo
    Aksentijevic, Dunja
    Sundier, Stephanie Y.
    Robb, Ellen L.
    Logan, Angela
    Nadtochiy, Sergiy M.
    Ord, Emily N. J.
    Smith, Anthony C.
    Eyassu, Filmon
    Shirley, Rachel
    Hu, Chou-Hui
    Dare, Anna J.
    James, Andrew M.
    Rogatti, Sebastian
    Hartley, Richard C.
    Eaton, Simon
    Costa, Ana S. H.
    Brookes, Paul S.
    Davidson, Sean M.
    Duchen, Michael R.
    Saeb-Parsy, Kourosh
    Shattock, Michael J.
    Robinson, Alan J.
    Work, Lorraine M.
    Frezza, Christian
    Krieg, Thomas
    Murphy, Michael P.
    [J]. NATURE, 2014, 515 (7527) : 431 - +
  • [3] DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS
    DAVIS, BJ
    [J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1964, 121 (A2) : 404 - &
  • [4] The role of promoter methylation in the regulation of genes encoding succinate dehydrogenase in maize seedlings
    Eprintsev, A. T.
    Fedorin, D. N.
    Selivanova, N. V.
    Wu, T. L.
    Makhmud, A. S.
    Popov, V. N.
    [J]. RUSSIAN JOURNAL OF PLANT PHYSIOLOGY, 2012, 59 (03) : 299 - 306
  • [5] Role of differential expression of sdh1-1 and sdh1-2 genes in alteration of isoenzyme composition of succinate dehydrogenase in germinating maize seeds
    Eprintsev, A. T.
    Fedorin, D. N.
    Selivanova, N. V.
    Akhmad, G. A.
    Popov, V. N.
    [J]. BIOLOGY BULLETIN, 2010, 37 (03) : 268 - 276
  • [6] Eprintsev A.T, 2007, GLIOKSILATNYI TSIKL
  • [7] Fedorin D. N., 2016, SORBTS KHROMATOGR PR, V16, P280
  • [8] The four subunits of mitochondrial respiratory complex II are encoded by multiple nuclear genes and targeted to mitochondria in Arabidopsis thaliana
    Figueroa, P
    Léon, G
    Elorza, A
    Holuigue, L
    Araya, A
    Jordana, X
    [J]. PLANT MOLECULAR BIOLOGY, 2002, 50 (4-5) : 725 - 734
  • [9] Three different genes encode the iron-sulfur subunit of succinate dehydrogenase in Arabidopsis thaliana
    Figueroa, P
    León, G
    Elorza, A
    Holuigue, L
    Jordana, X
    [J]. PLANT MOLECULAR BIOLOGY, 2001, 46 (02) : 241 - 250
  • [10] Gaal O., 1980, ELECTROPHORESIS SEPA
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