RPLC-Ion-Trap-FTMS Method for Lipid Profiling of Plasma: Method Validation and Application to p53 Mutant Mouse Model

被引：142

作者：

Hu, Chunxiu ^{[1
,2
]}

van Dommelen, Judith ^{[1
]}

van der Heijden, Rob ^{[1
]}

Spijksma, Gerwin ^{[1
]}

Reijmers, Theo H. ^{[1
]}

Wang, Mei ^{[3
,4
]}

Slee, Elizabeth ^{[5
]}

Lu, Xin ^{[5
]}

Xu, Guowang ^{[2
]}

van der Greef, Jan ^{[1
,3
,4
]}

Hankemeier, Thomas ^{[1
]}

机构：

[1] Leiden Univ, LACDR, Div Analyt Biosci, NL-2300 RA Leiden, Netherlands

[2] Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

[3] SU BioMed, NL-3700 AJ Zeist, Netherlands

[4] TNO Qual Life, NL-3700 AJ Zeist, Netherlands

[5] Univ Oxford, Ludwig Inst Canc Res, Oxford OX3 7DQ, England

来源：

JOURNAL OF PROTEOME RESEARCH | 2008年 / 7卷 / 11期

关键词：

Lipidomics; Lipid Profiling; Metabolite Profiling; Metabolomics; FTMS; p53 Mouse Model;

D O I：

10.1021/pr800373m

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometric method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C-8 column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single LC-MS run. The method was fully validated and the analytical characteristics such as linearity (R-2, 0.994-1.000), limit of detection (0.08-1.28 mu g/mL plasma), repeatability (RSD, 2.7-7.9%) and intermediate precision (RSD, 2.7-15.6%) were satisfactory. The method was successfully applied to p53 mutant mice plasma for studying some phenotypic effects of p53 expression.

引用

页码：4982 / 4991

页数：10

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