Determination of lactate dehydrogenase at a modified carbon fibre electrode

The cyclic voltammetric electropolymerization of multilayer coatings of the dye methylene green on carbon fibre electrode is reported. The modified carbon fibre electrode was used to the assay of lactate dehydrogenase (LDH). Under the concentration of nicotinamide adenine dinucleotide (NAD(+)) being 6.0 x 10(-4) moI/L, of lactate being 5.0 x 10(-3) moI/L, the modified electrode could detect LDH sensitively at + 0.10 V( vs. SCE) with linear response in the range of 15 U/mL to 240 U/mL. The detection limit was 10 U/mL. Evidence is presented to show the good stability of the modified electrode.