A hyperosmotic stimulus regulates intracellular pH, calcium, and S-100 protein levels in avian chondrocytes

被引:20
作者
Dascalu, A
Korenstein, R
Oron, Y
Nevo, Z
机构
[1] TEL AVIV UNIV,SACKLER FAC MED,DEPT PHYSIOL & PHARMACOL,IL-69978 TEL AVIV,ISRAEL
[2] TEL AVIV UNIV,SACKLER FAC MED,DEPT CLIN BIOCHEM,IL-69978 TEL AVIV,ISRAEL
关键词
D O I
10.1006/bbrc.1996.1514
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cartilage is exposed to mechanical loads, generating at the level of single chondrocytes a hyperosmotic stimulus (HOS). The direct effect of HOS on second messenger pathways in avian chondrocytes was evaluated by fluorimetric and image analysis techniques. HOS caused an immediate intracellular acidification of 0.07 +/- 0.02 pH units (n=7), followed by an initial pH recovery rate of 0.033 +/- 0.04 pH units/min towards the pre-stimulus baseline values. Concomitantly, the intracellular calcium ([Ca2+](i)) responded with a transient rise from baseline value of 84.7 +/- 7.4 nM to peak level of 403.1 +/- 51.0 nM (n=16, p<0.001). The calcium response was abolished by two calmodulin inhibitors chlorpromazine and W-7. Since these inhibitors are known to be specific ligands of a S-100 protein, its intracellular staining was determined following HOS. The amount of immunodetectable S-100 protein was significantly increased following exposure to HOS (p<0.05), and did not require an increase of [Ca2+](i). It appears that compression of cartilage is transduced into HOS of chondrocytes, and further elicits its effect through transient intracellular elevation of protons and calcium ions accompanied by increased staining of S-100 protein. (C) 1996 Academic Press, Inc.
引用
收藏
页码:368 / 373
页数:6
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