Pentoxifylline influences drug transport activity of P-glycoprotein and decreases mdr1 gene expression in multidrug resistant mouse leukemic L1210/VCR cells

被引：0

作者：

Drobná, Z

Stein, U

Walther, W

Barancík, M

Breier, A

机构：

[1] Slovak Acad Sci, Inst Mol Physiol & Genet, Bratislava 83334, Slovakia

[2] Heart Res Inst, Bratislava, Slovakia

[3] Max Delbruck Ctr Mol Med, Berlin, Germany

来源：

GENERAL PHYSIOLOGY AND BIOPHYSICS | 2002年 / 21卷 / 01期

关键词：

pentoxifylline; doxorubicin; mdr1; mRNA; P-glycoprotein; multidrug resistance; vincristine; L1210; cells;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of pentoxifylline (PTX) on intracellular accumulation of doxorobicin (DOX), DOX cytotoxicity and expression of Pgp in multidrug resistant L1210/VCR cell line were investigated. PTX (100 mg/l) was able to enhance the DOX accumulation in resistant cells. The maximum intracellular levels of DOX were reached after treatment with PTX for 24 hours (total duration of PTX-treatment was 72 hours). The levels of mdr1 mRNA (measured by RT-PCR method) were decreased 2-fold in the presence of 100 mg/l PTX (minimum reached within 48 hours) in comparison to control cells.

引用

页码：103 / 109

页数：7

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