NOVEL BIOREACTORS FOR OSTEOCHONDRAL TISSUE ENGINEERING

Tissue engineering is a new approach for articular cartilage repair, but the integration of engineered cartilage into the host subchondral bone is a major problem. One approach for solving this problem is to make osteochondral tissue engineering instead of cartilage tissue engineering only. The aim of the present paper was to describe two patented newly designed bioreactors for tissue-engineered osteochondral graft. The first bioreactor is double-chamber bioreactor, which is made of glass and is completely transparent. The whole system consists of one chamber for culture of chondrocytes and the other chamber for osteoblast culture. One important role for this bioreactor is to co-culture osteoblasts and chondrocytes at the same time in a biphasic scaffold. The bioreactor is modified from spinner flasks. The stirring of the magnetic bars provides medium mixing and mechanical stimulations for the cells. The second bioreactor is modified from perfusion chamber. The driven force of the medium flow is produced by siphon phenomenon. The bioreactor is composed with two parts. The first part is a modified siphon tube which can hold the biphasic scaffold for osteochondral tissue engineering. The second part is a medium reservoir bottle, which contains large amount of medium and can connect to multiple siphon tubes at the same time. The medium reservoir bottle is placed higher than the siphon tubes. The gravity will drive medium into the siphon tubes. The siphon phenomenon will make the cell-seeded scaffold covered with the medium. When the height of medium reach the height of outflow tube of the siphon tube, the medium will drain out, and the scaffold will be exposed to the air in incubator, which provides oxygen exposure. Then the gravity will make the medium refill again, the scaffold will be immersed in medium until next cycle of medium drainage out. The curve shape of the siphon tube will prevent backward bacteria contamination. The flow of the medium from reservoir through the siphon tube will produce an effect like traditional perfusion chamber bioreactor; however no power supply is necessary in this system.