Genome-Wide Analysis and Molecular Characterization of Heat Shock Transcription Factor Family in Glycine max

被引：58
作者：
Chung, Eunsook [1 ]
Kim, Kyoung-Mi [1 ]
Lee, Jai-Heon [1 ]
机构：
[1] Dong A Univ, Ctr Silver Bio Industrializat, Coll Nat Resources & Life Sci, Pusan 604714, South Korea
来源：
JOURNAL OF GENETICS AND GENOMICS | 2013年 / 40卷 / 03期
基金：
新加坡国家研究基金会;
关键词：
Abiotic stress; Arabidopsis thaliana; Expression patterns; Glycine max; Heat shock transcription factor; Phylogenetic analysis; DNA-BINDING DOMAIN; INTRACELLULAR-DISTRIBUTION; DEVELOPMENTAL REGULATION; FUNCTIONAL-ANALYSIS; ACTIVATOR FUNCTION; STRESS RESPONSE; NUCLEAR IMPORT; FACTOR DREB2A; FACTOR HSFA3; GENE;
D O I：
10.1016/j.jgg.2012.12.002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Heat shock transcription factors (Hsfs) play an essential role on the increased tolerance against heat stress by regulating the expression of heat-responsive genes. In this study, a genome-wide analysis was performed to identify all of the soybean (Glycine max) GmHsf genes based on the latest soybean genome sequence. Chromosomal location, protein domain, motif organization, and phylogenetic relationships of 26 non-redundant GmHsf genes were analyzed compared with AtHsfs (Arabidopsis thaliana Hsfs). According to their structural features, the predicted members were divided into the previously defined classes A-C, as described for AtHsfs. Transcript levels and subcellular localization of five GmHsfs responsive to abiotic stresses were analyzed by real-time RT-PCR. These results provide a fundamental clue for understanding the complexity of the soybean GmHsf gene family and cloning the functional genes in future studies.
引用
收藏
页码：127 / 135
页数：9
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