Trapping DNA near a Solid-State Nanopore

被引：43

作者：

Vlassarev, Dimitar M. ^{[1
]}

Golovchenko, Jene A. ^{[1
,2
]}

机构：

[1] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

[2] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

来源：

BIOPHYSICAL JOURNAL | 2012年 / 103卷 / 02期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

MOLECULES; DISCRIMINATION; TRANSLOCATION; MEMBRANE; GRAPHENE; GLASS;

D O I：

10.1016/j.bpj.2012.06.008

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We demonstrate that voltage-biased solid-state nanopores can transiently localize DNA in an electrolyte solution. A double-stranded DNA (dsDNA) molecule is trapped when the electric field near the nanopore attracts and immobilizes a non-end segment of the molecule across the nanopore orifice without inducing a folded molecule translocation. In this demonstration of the phenomenon, the ionic current through the nanopore decreases when the dsDNA molecule is trapped by the nanopore. By contrast, a translocating dsDNA molecule under the same conditions causes an ionic current increase. We also present finite-element modeling results that predict this behavior for the conditions of the experiment.

引用

页码：352 / 356

页数：5

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