Effects of species, plant part, and plant age on the n-alkane concentrations in the cuticular wax of pasture plants

Plants of the pasture species Phalaris aquatica cv. Sirosa, Lolium perenne cv. Victorian Trifolium repens cv. Irrigation White, T. subterraneum ssp. subterraneum cv. Mt Barker, T. subterraneum ssp. yanninicum cv. Trikkala, and Medicago sativa, cv. Siriver were grown under controlled glasshouse conditions. At weekly intervals, 6 plants of each species were harvested and dissected into their component plant parts. The concentrations of n-alkanes in plant parts from all species were then estimated using gas chromatography. Results confirmed earlier studies that alkanes with odd-numbered carbon chains were predominant in cuticular wax, especially C27, C29, C31, and C33 alkanes. For the individual alkanes C25-C33, differences between species accounted for 85% of the total variance in alkane concentration. Calculation of similarity coefficients indicated that the greatest similarities in the pattern of alkane concentrations occurred either between plant parts within a species or between the same plant part in closely related species. Multivariate statistical analysis using canonical variates analyses indicated that despite these similarities, it would still be possible to distinguish both plant species and plant parts in mixtures of these components. In particular, an examination of hypothetical perennial ryegrass-white clover or phalaris-subterranean clover pastures demonstrated that all fractions of all species would be likely to be distinguishable. The results are discussed in relation to the use of herbage and faecal alkane concentrations in least-squares estimates of the composition of the diet of the grazing animal.