Hydroxyapatite/regenerated silk fibroin scaffold-enhanced osteoinductivity and osteoconductivity of bone marrow-derived mesenchymal stromal cells

被引：49

作者：

Jiang, Jia ^{[1
]}

Hao, Wei ^{[2
]}

Li, Yuzhuo ^{[1
]}

Yao, Jinrong ^{[2
]}

Shao, Zhengzhong ^{[2
]}

Li, Hong ^{[1
]}

Yang, Jianjun ^{[1
]}

Chen, Shiyi ^{[1
]}

机构：

[1] Fudan Univ, Huashan Hosp, Dept Sports Med & Arthroscopy, Fudan Univ Sports Med Ctr, Shanghai 200040, Peoples R China

[2] Fudan Univ, Dept Macromol Sci, Natl Key Lab Mol Engn Polymers, Adv Mat Lab, Shanghai 200433, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2013年 / 35卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Bone tissue engineering; Hydroxyapatite; Osteoconductivity; Osteoinductivity; Regenerated silk fibroin; MICROSPHERES; BMP-2;

D O I：

10.1007/s10529-012-1121-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel hydroxyapatite/regenerated silk fibroin scaffold was prepared and investigated for its potential to enhance both osteoinductivity and osteoconductivity of bone marrow-derived mesenchymal stromal cells in vitro. Approx. 12.4 +/- A 0.06 % (w/w) hydroxyapatite was deposited onto the scaffold, and cell viability and DNA content were significantly increased (18.5 +/- A 0.6 and 33 +/- A 1.2 %, respectively) compared with the hydroxyapatite scaffold after 14 days. Furthermore, alkaline phosphatase activity in the novel scaffold increased 41 +/- A 2.5 % after 14 days compared with the hydroxyapatite scaffold. The data indicate that this novel hydroxyapatite/regenerated silk fibroin scaffold has a positive effect on osteoinductivity and osteoconductivity, and may be useful for bone tissue engineering.

引用

页码：657 / 661

页数：5

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