7-Triazolylcoumarin-based fluorescent tag system for stepwise, comparative assessment of small molecule microarrays

被引：14
作者：
Jeon, Moon-Kook [1 ]
Kang, Myoung-Ku [1 ,2 ]
Park, Koon Ha [2 ]
机构：
[1] Korea Res Inst Chem Technol, Div Drug Discovery Res, Taejon 305600, South Korea
[2] Chungnam Natl Univ, Dept Chem, Taejon 305764, South Korea
来源：
TETRAHEDRON | 2012年 / 68卷 / 30期
关键词：
Small molecule microarray; Tag; Fluorescence; Triazolylcoumarin; Fluorous; PHASE SYNTHETIC METHOD; ORAL BIOAVAILABILITY; FLUOROUS TAGS; DISCOVERY; EMISSION; LIGANDS; PEPTIDE; COMPLEX; BIOLOGY; TOOLS;
D O I：
10.1016/j.tet.2012.05.018
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
The potential use of a fluorescent tag system based on 7-(1H-1,2,3-triazol-4-yl)coumarin fluorophore having a fluorous moiety and a polyethylene glycol (PEG) spacer at opposite ends as a tool for a stepwise and comparative evaluation of the fabrication process of small molecule microarrays was illustrated by the qualitative analysis of the results of the fluorescence detection obtained from the microarray experiments using the tagged biotins and streptavidin-Cy3 (and avidin-Cy5) as the binding partners. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：6038 / 6053
页数：16
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