Homogeneous fluorescent immunoassay for the simultaneous detection of chloramphenicol and amantadine via the duplex FRET between carbon dots and WS2 nanosheets

被引：45

作者：

Dong, Baolei ^{[1
]}

Li, Hongfang ^{[1
]}

Sun, Jiefang ^{[2
]}

Mari, Ghulam Mujtaba ^{[1
]}

Ai, Jun ^{[3
]}

Han, Diangang ^{[3
]}

Yu, Xuezhi ^{[1
]}

Wen, Kai ^{[1
]}

Shen, Jianzhong ^{[1
]}

Wang, Zhanhui ^{[1
]}

机构：

[1] China Agr Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing Lab Food Qual & Safety, Beijing 100193, Peoples R China

[2] Beijing Res Ctr Prevent Med, Beijing Key Lab Diagnost & Traceabil Food Poisoni, Beijing 100013, Peoples R China

[3] Technol Ctr Kunming Customs, Kunming 650299, Yunnan, Peoples R China

来源：

FOOD CHEMISTRY | 2020年 / 327卷

基金：

欧盟地平线“2020”;

关键词：

Homogeneous immunoassay; Carbon dots; WS2; nanosheets; Fluorescence resonance energy transfer; Chloramphenicol; Amantadine; IMMUNOCHROMATOGRAPHIC ASSAY; SENSITIVE DETECTION; FOOD; RESIDUES; SPECTRUM; 1T-WS2; GREEN;

D O I：

10.1016/j.foodchem.2020.127107

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Herein, we proposed a duplex and homogeneous fluorescent immunoassay for the simultaneous detection of amantadine (AMD) and chloramphenicol (CAP) residue in chicken breast with both high sensitivity and short assay time. The immunoassay was based on the fluorescence resonance energy transfer (FRET) between hapten-labeled carbon dots (CDs) and antibody-modified WS2 nanosheets. To achieve the duplex FRET, polyethyleneimine-functionalized blue and green emissive CDs with separated emission were synthesized via a one-pot hydrothermal method and directly coupled with the haptens of AMD and CAP, serving as the energy donors. The antibodies were modified on the surface of WS2 nanosheets with high quenching efficiency to construct the energy acceptor. The specific immunoreaction could trigger the efficient FRET between the donors and the acceptors, causing the fluorescence quenching of CDs. The developed immunoassay was applied to simultaneously detect AMD and CAP, having the detection limit of 0.10 ng g(-1) and 0.06 ng g(-1), respectively.

引用

页数：9

共 38 条

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