Exosomal Transfer of Vasorin Expressed in Hepatocellular Carcinoma Cells Promotes Migration of Human Umbilical Vein Endothelial Cells

被引：92

作者：

Huang, Aixue ^{[1
]}

Dong, Jie ^{[1
]}

Li, Shaohua ^{[1
]}

Wang, Chaonan ^{[1
]}

Ding, Hongmei ^{[1
]}

Li, Hui ^{[1
]}

Su, Xueting ^{[1
]}

Ge, Xingfeng ^{[1
]}

Sun, Leqiao ^{[1
]}

Bai, Chenjun ^{[1
]}

Shen, Xuelian ^{[1
]}

Fang, Tao ^{[1
]}

Li, Jie ^{[1
]}

Shao, Ningsheng ^{[1
]}

机构：

[1] Beijing Inst Basic Med Sci, Beijing 100850, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES | 2015年 / 11卷 / 08期

关键词：

Vasorin; exosomes; endocytosis; angiogenesis; tumor metastasis; TARGET; INTERNALIZATION; BIOMARKERS; PROTEINS; GROWTH; ACID; RAT;

D O I：

10.7150/ijbs.11943

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vasorin (VASN) is a type I transmembrane protein that plays important roles in tumor development and vasculogenesis. In this paper, we showed that VASN could be a key mediator of communication between tumor cells and endothelial cells. We confirmed for the first time that HepG2-derived VASN can be transferred to human umbilical vein endothelial cells (HUVECs) via receptor mediated endocytosis of exosomes, at least in part through HSPGs. The HepG2-derived VASN containing exosomes promote migration of recipient HUVECs cells. Our results identify a novel pathway by which a functional protein expressed in tumor cells affects the biological fate of endothelial cells via exosomes.

引用

页码：961 / 969

页数：9

共 35 条

[11] Selective transfer of exosomes from oligodendrocytes to microglia by macropinocytosis [J].

Fitzner, Dirk ;

Schnaars, Mareike ;

van Rossum, Denise ;

Krishnamoorthy, Gurumoorthy ;

Dibaj, Payam ;

Bakhti, Mostafa ;

Regen, Tommy ;

Hanisch, Uwe-Karsten ;

Simons, Mikael .

JOURNAL OF CELL SCIENCE, 2011, 124 (03) :447-458

[12] Targeting the tumor stroma in hepatocellular carcinoma [J].

Heindryckx, Femke ;

Gerwins, Par .

WORLD JOURNAL OF HEPATOLOGY, 2015, 7 (02) :165-176

[13] Proteomic Profiling of γ-Secretase Substrates and Mapping of Substrate Requirements [J].

Hemming, Matthew L. ;

Elias, Joshua E. ;

Gygi, Steven P. ;

Selkoe, Dennis J. .

PLOS BIOLOGY, 2008, 6 (10) :2314-2328

[14] Micromechanical control of cell-cell interactions [J].

Hui, Elliot E. ;

Bhatia, Sangeeta N. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (14) :5722-5726

[15] Vasorin, a transforming growth factor β-binding protein expressed in vascular smooth muscle cells, modulates the arterial response to injury in vivo [J].

Ikeda, Y ;

Imai, Y ;

Kumagai, H ;

Nosaka, T ;

Morikawa, Y ;

Hisaoka, T ;

Manabe, I ;

Maemura, K ;

Nakaoka, T ;

Imamura, T ;

Miyazono, K ;

Komuro, I ;

Nagai, R ;

Kitamura, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (29) :10732-10737

[16] Exosomes: Vehicles of Intercellular Signaling, Biomarkers, and Vectors of Cell Therapy [J].

Kourembanas, Stella .

ANNUAL REVIEW OF PHYSIOLOGY, VOL 77, 2015, 77 :13-27

[17] Expression of vasorin (Vasn) during embryonic development of the mouse [J].

Krautzberger, A. Michaela ;

Kosiol, Barbara ;

Scholze, Manuela ;

Schrewe, Heinrich .

GENE EXPRESSION PATTERNS, 2012, 12 (5-6) :167-171

[18] Identification of cardiac-related circulating microRNA profile in human chronic heart failure [J].

Li, Huaping ;

Fan, Jiahui ;

Yin, Zhongwei ;

Wang, Feng ;

Chen, Chen ;

Wang, Dao Wen .

ONCOTARGET, 2016, 7 (01) :33-45

[19] Prostate Tumor-Derived Exosomes Down-Regulate NKG2D Expression on Natural Killer Cells and CD8+ T Cells: Mechanism of Immune Evasion [J].

Lundholm, Marie ;

Schroder, Mona ;

Nagaeva, Olga ;

Baranov, Vladimir ;

Widmark, Anders ;

Mincheva-Nilsson, Lucia ;

Wikstrom, Pernilla .

PLOS ONE, 2014, 9 (09)

[20] ADAM17 (TACE) regulates TGFβ signaling through the cleavage of vasorin [J].

Malapeira, J. ;

Esselens, C. ;

Bech-Serra, J. J. ;

Canals, F. ;

Arribas, J. .

ONCOGENE, 2011, 30 (16) :1912-1922

← 1 2 3 4 →