Analysis of human papillomavirus and Epstein-Barr virus infection and aberrant death-associated protein kinase methylation in high-grade squamous intraepithelial lesions

被引：14

作者：

Lattario, F. ^{[1
]}

Furtado, Y. L. ^{[2
]}

Fonseca, R. ^{[2
]}

Silveira, F. A. ^{[2
]}

Do Val, I. C. ^{[2
]}

Almeida, G. ^{[2
]}

Carvalho, M. G. C. ^{[1
]}

机构：

[1] Inst Biofis Carlos Chagas Filho, Gene Express Control Lab, UFRJ, CCS, BR-21949900 Rio De Janeiro, RJ, Brazil

[2] Univ Fed Rio de Janeiro, Inst Gynecol, Rio De Janeiro, Brazil

来源：

INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER | 2008年 / 18卷 / 04期

关键词：

cervix uteri; DAPK; EBV; HPV; methylation;

D O I：

10.1111/j.1525-1438.2007.01060.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

This study was conducted to investigate the presence of Epstein-Barr virus (EBV) and human papillomavirus (HPV) and the promoter methylation status of the death-associated protein kinase (DAPK) gene in high-grade intraepithelial lesions. Viral infection was analyzed using polymerase chain reaction (PCR), and promoter methylation status was evaluated using chemical modification by sodium bisulfite followed by PCR. A total of 24 samples were studied. HPV was detected in 16.6%, EBV in 16.6%, and HPV/EBV coinfection in 16.6%. No virus infection was detected in 50% of the samples studied. DAPK promoter methylation was observed in 29.2% of the analyzed samples. There was no significant correlation between DAPK methylation and viral infection. DAPK methylation was detected in 28% of HPV-positive lesions, in 28% of HPV- and EBV-positive lesions, and in 44% (3/7) of the samples without viral infection. There was no observed methylation in samples with isolated EBV infection. In DAPK unmethylated samples, HPV infection was found in 12%, EBV infection in 23%, HPV/EBV coinfection in 12%, and an absence of HPV and EBV infection in 53%. The promoter methylation of the DAPK gene is an important event during carcinogenesis and may have potential clinical application as a marker for the progression and prognosis of cancer.

引用

页码：785 / 789

页数：5