Purification and characterization of Cry1Ac toxin binding proteins from the brush border membrane of Helicoverpa armigera midgut

被引：17
作者：
Liao, CY
Trowell, SC
Akhurst, R
机构：
[1] CSIRO Entomol, Canberra, ACT 2601, Australia
[2] Australian Natl Univ, Div Biochem & Mol Biol, Canberra, ACT 2601, Australia
来源：
CURRENT MICROBIOLOGY | 2005年 / 51卷 / 06期
关键词：
D O I：
10.1007/s00284-005-0051-9
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Several Cry1Ac binding proteins from midgut of Helicoverpa armigera were purified using toxin-affinity chromatography. Enzyme assays showed that the purified proteins had strong aminopeptidase activity. The N-terminal sequences confidently identified a 124-kDa binding protein as an aminopeptidase N (APN), and some similarity suggests that a 162-kDa binding protein may also be an APN. Two minor binding proteins were not characterized.
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收藏
页码：367 / 371
页数：5
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