EXTRACTION STUDIES OF PODOPHYLLUM HEXANDRUM USING CONVENTIONAL AND NONCONVENTIONAL METHODS BY HPLC-UV-DAD

The composition of lignans extracted from Podophyllum hexandrum rhizomes was studied by sequential extraction with supercritical CO2, ethyl acetate modified CO2 and methanol modified CO2. The results were compared with the extracts obtained by Accelerated Solvent Extraction (ASE) and soxhlet's methods. The lignan contents comprised of Podophyllotoxin, deoxypodophyllotoxin, 4-demethylpodophyllotoxin, Picropodophyllotoxin, Isopicropodophyllotoxin, Podophllotoxin--D-glucopyranoside, and 4-demethylpodophyllotoxin -D-glucopyranoside in the extracts of Podophyllum hexandrum rhizomes obtained by different methods was studied. There was a variation in the concentration of lignan in the extracts obtained by different methods of extraction. Podophyllotoxin formed the major component (36.55%) of the extract obtained by SFE and picropodophyllotoxin, isopicropodophyllotoxin, deoxypodophyllotoxin, and 4-demethylpodophyllotoxin were present in 6.82, 1.51, 1.46, and 0.85%, respectively, whereas 4-demethylpodophyllotoxin -D-glucopyranoside was 1.89% in the extract obtained by ASE, which is higher than SFE extracts. Soxhlet's derived extract contains better concentration of isopicropodophyllotoxin (24.49%) than SFE. A simple, isocratic, and reliable analytical HPLC-UV (DAD) method was developed for simultaneous identification and quantification of different seven lignans in the extracts of different extraction techniques.