Multiplex PCR for the discrimination of A-fumigatus, A-flavus, A-niger and A-terreus

被引：24

作者：

Logotheti, M. ^{[1
]}

Kotsovili-Tseleni, A. ^{[1
]}

Arsenis, G. ^{[1
]}

Legakis, N. I. ^{[1
]}

机构：

[1] Univ Athens, Dept Microbiol, Sch Med, GR-10679 Athens, Greece

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2009年 / 76卷 / 02期

关键词：

Aspergillus; Multiplex PCR; Aspergillopepsins; ITS;

D O I：

10.1016/j.mimet.2008.10.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aspergillus pathogens usually infect immunocompromised patients with lethal outcome. We report a multiplex PCR assay for the discrimination of the most frequent Aspergillus pathogens, A. fumigatus, A. flavus, A. niger and A. terreus, through distinct amplicons of 250 bp, 200 bp, 150 bp and 450 bp respectively, derived from the rDNA gene of A. terreus and the aspergillopepsin genes of the remaining species. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：209 / 211

页数：3

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