Requirement of guanosine triphosphate-bound Ran for signal-mediated nuclear protein export

被引：167
作者：
Richards, SA
Carey, KL
Macara, IG
机构：
[1] UNIV VIRGINIA,HLTH SCI CTR,CTR CELL SIGNALING,CHARLOTTESVILLE,VA 22908
[2] UNIV VERMONT,DEPT PATHOL,BURLINGTON,VT 05405
来源：
SCIENCE | 1997年 / 276卷 / 5320期
关键词：
D O I：
10.1126/science.276.5320.1842
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A leucine-rich nuclear export signal (NES) allows rapid export of proteins from cell nuclei. Microinjection studies revealed a role for the guanosine triphosphatase (GTPase) Ran in NES-mediated export. Nuclear injection of a Ran mutant (Thr(24)-->Asn) blocked protein export but not import, whereas depletion of the Ran nucleotide exchange factor RCC1 blocked protein import but not export. However, injection of Ran GTPase-activating protein (RanGAP) into RCC1-depleted cell nuclei inhibited export. Coinjection with Ran mutants insensitive to RanGAP prevented this inhibition. Therefore, NES-mediated protein export appears to require a Ran-GTP complex but does not require Ran-dependent GTP hydrolysis.
引用
收藏
页码：1842 / 1844
页数：3
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