Highly sensitive detection for stimulants using an aptamer functionalised gold nanoparticles

被引：4

作者：

Shi, Haixia ^{[1
,2
]}

Li, Qin ^{[3
]}

Li, Raoqi ^{[3
]}

Hu, Ti ^{[4
]}

Yao, Lei ^{[1
]}

Gao, Li ^{[3
]}

机构：

[1] Beijing Sport Univ, Coll Educ, Beijing 100084, Peoples R China

[2] Dalian Jiaotong Univ, PE Dept, Dalian 116028, Peoples R China

[3] Jiangsu Univ, Inst Life Sci, Zhenjiang 212013, Peoples R China

[4] Beijing Normal Univ, Coll PE & Sports, Beijing 100875, Peoples R China

来源：

MATERIALS TECHNOLOGY | 2015年 / 30卷 / B3期

关键词：

Cocaine detection; Aptamer; Gold nanoparticles; Highly sensitive detection; Stimulants; ROLLING-CIRCLE AMPLIFICATION; COCAINE DETECTION; ENERGY-TRANSFER; SENSOR; APTASENSOR; SINGLE; URINE;

D O I：

10.1179/17535557B15Y.000000003

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

In this paper, a simple, highly sensitive, and cost-effective method was described for cocaine detection as an example in the stimulants using an aptamer-functionalised gold nanoparticles (Au NPs). Cocaine-specific aptamer is single-strand DNA sequence that can specifically bind to cocaine. Carboxyfluorescein (FAM)-labelled aptamer was adsorbed on the surface of Au NPs. Then FAM was quenched owing to the close proximity between aptamer and Au NPs. Cocaine can be detected after the fluorescence of FAM was restored by detaching from the surface of Au NPs. The limit of detection (LOD) was 3 pM. The results show this sensitive method is promising for the detection of stimulants.

引用

页码：B178 / B181

页数：4

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