High-performance and site-directed in utero electroporation by a triple-electrode probe

被引:99
作者
dal Maschio, Marco [1 ]
Ghezzi, Diego [1 ]
Bony, Guillaume [1 ]
Alabastri, Alessandro [2 ]
Deidda, Gabriele [1 ]
Brondi, Marco [3 ,4 ,5 ]
Sato, Sebastian Sulis [3 ,4 ,6 ]
Zaccaria, Remo Proietti [2 ]
Di Fabrizio, Enzo [2 ,7 ]
Ratto, Gian Michele [3 ,4 ,5 ]
Cancedda, Laura [1 ]
机构
[1] Ist Italiano Tecnol, Dept Neurosci & Brain Technol, I-16163 Genoa, Italy
[2] Ist Italiano Tecnol, Dept Nanostruct, I-16163 Genoa, Italy
[3] Ist Nanosci CNR, Lab NEST, I-56120 Pisa, Italy
[4] Scuola Normale Super Pisa, I-56120 Pisa, Italy
[5] Ist Neurosci CNR, I-56124 Pisa, Italy
[6] IIT NEST, Ctr Nanotechnol Innovat, I-56120 Pisa, Italy
[7] Magna Graecia Univ Catanzaro, BIONEM Lab, I-88100 Catanzaro, Italy
关键词
REGULATES NEURONAL POLARIZATION; CENTRAL-NERVOUS-SYSTEM; GENE-TRANSFER; VIVO ELECTROPORATION; DIELECTRIC-PROPERTIES; BIOLOGICAL TISSUES; PYRAMIDAL NEURONS; AXON FORMATION; BRAIN; MIGRATION;
D O I
10.1038/ncomms1961
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In utero electroporation is a powerful tool to transfect and manipulate neural-precursor cells of the rodent parietal cortex and their progeny in vivo. Although this technique can potentially target numerous brain areas, reliability of transfection in some brain regions is low or physical access is limited. Here we present a new in utero electroporation configuration based on the use of three electrodes, the relative position and polarities of which can be adjusted. The technique allows easy access and exceedingly reliable monolateral or bilateral transfection at brain locations that could only be sporadically targeted before. By improvement in the efficiency of the electrical field distribution, demonstrated here by a mathematical simulation, the multi-electrode configuration also extends the developmental timeframe for reliable in utero electroporation, allowing for the first time specific transfection of Purkinje cells in the rat cerebellum.
引用
收藏
页数:11
相关论文
共 44 条
[1]   Lateral competition for cortical space by layer-specific horizontal circuits [J].
Adesnik, Hillel ;
Scanziani, Massimo .
NATURE, 2010, 464 (7292) :1155-U71
[2]   AMP-Activated Protein Kinase Regulates Neuronal Polarization by Interfering with PI 3-Kinase Localization [J].
Amato, Stephen ;
Liu, Xiuxin ;
Zheng, Bin ;
Cantley, Lewis ;
Rakic, Pasko ;
Man, Heng-Ye .
SCIENCE, 2011, 332 (6026) :247-251
[3]   Sublayer-specific microcircuits of corticospinal and corticostriatal neurons in motor cortex [J].
Anderson, Charles T. ;
Sheets, Patrick L. ;
Kiritani, Taro ;
Shepherd, Gordon M. G. .
NATURE NEUROSCIENCE, 2010, 13 (06) :739-U116
[4]   Excitatory GABA action is essential for morphological maturation of cortical neurons in vivo [J].
Cancedda, Laura ;
Fiumelli, Hubert ;
Chen, Karen ;
Poo, Mu-ming .
JOURNAL OF NEUROSCIENCE, 2007, 27 (19) :5224-5235
[5]   Ephrin-As guide the formation of functional maps in the visual cortex [J].
Cang, JH ;
Kaneko, M ;
Yamada, J ;
Woods, G ;
Stryker, MP ;
Feldheim, DA .
NEURON, 2005, 48 (04) :577-589
[6]   In vivo electroporation of the central nervous system: A non-viral approach for targeted gene delivery [J].
De Vry, Jochen ;
Martinez-Martinez, Pilar ;
Losen, Mario ;
Temel, Yasin ;
Steckler, Thomas ;
Steinbusch, Harry W. M. ;
De Baets, Marc H. ;
Prickaerts, Jos .
PROGRESS IN NEUROBIOLOGY, 2010, 92 (03) :227-244
[7]  
DOTTI CG, 1988, J NEUROSCI, V8, P1454
[8]  
Elias Laura, 2007, J Vis Exp, P235, DOI 10.3791/235
[9]   Cdk5-Mediated Phosphorylation of Axin Directs Axon Formation during Cerebral Cortex Development [J].
Fang, Wei-Qun ;
Ip, Jacque P. K. ;
Li, Rui ;
Ng, Yu Pong ;
Lin, Sheng-Cai ;
Chen, Yu ;
Fu, Amy K. Y. ;
Ip, Nancy Y. .
JOURNAL OF NEUROSCIENCE, 2011, 31 (38) :13613-13624
[10]   Neocortex patterning by the secreted signaling molecule FGF8 [J].
Fukuchi-Shimogori, T ;
Grove, EA .
SCIENCE, 2001, 294 (5544) :1071-1074