A novel activatable AIEgen fluorescent probe for peroxynitrite detection and its application in EC1 cells

被引：57

作者：

Han, Xiaojing ^{[1
]}

Yang, Xiaopeng ^{[1
]}

Zhang, Yongru ^{[1
]}

Li, Zipeng ^{[1
]}

Cao, Wenbo ^{[3
]}

Zhang, Di ^{[2
]}

Ye, Yong ^{[1
]}

机构：

[1] Zhengzhou Univ, Coll Chem, Green Catalysis Ctr, Zhengzhou 450001, Peoples R China

[2] Henan Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Zhengzhou 450002, Peoples R China

[3] Zhengzhou Univ, Sch Basic Med Sci, Zhengzhou 450001, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2020年 / 321卷

基金：

美国国家科学基金会;

关键词：

AIE fluorescent probe; Long emission wavelength; Peroxynitrite; Cell imaging; ENDOGENOUS PEROXYNITRITE; NITRIC-OXIDE; LIVING CELLS; RED;

D O I：

10.1016/j.snb.2020.128510

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Peroxynitrite (ONOO-), as one type of reactive nitrogen species (RNS), is closely related to multiple pathophysiological processes. Visualization of ONOO- in living system is essential for further study of its physiological functions. However, most existing ONOO- fluorescent probes continuously display the aggregation-caused quenching (ACQ). In present research, a new activatable AIEgen fluorescent probe QM-ONOO- for ONOO- recognition and imaging was proposed. Activation of the probe by ONOO-, induced aggregation of QM-OH. This sensor showed fast response time (< 4 min) and high sensitivity (DL, 27.5 nM), large Stock's shift (190 nm), long emission wavelength. Furthermore, the outcomes indicate that QM-ONOO- as an activatable AIEgen fluorescent sensor was robust chemical tool to detect endogenous peroxynitrite in living biological system.

引用

页数：6

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