Characterization of Methylophaga species by rRNA gene restriction patterns (ribotyping)

被引：3
|
作者：
Janvier, M [1 ]
Grimont, RAD [1 ]
Grimont, F [1 ]
机构：
[1] Inst Pasteur, INSERM, U389, Unite Enterobacteries, F-75724 Paris 15, France
来源：
SYSTEMATIC AND APPLIED MICROBIOLOGY | 1999年 / 22卷 / 03期
关键词：
Methylophaga; ribotyping; taxonomy; identification; environment;
D O I：
10.1016/S0723-2020(99)80045-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A total of 24 strains of Methylophaga, marine methylotrophic bacteria, were characterized by rRNA gene restriction patterns (ribotypes), after cleavage of total DNA with EcoRI or HindIII restriction endonuclease. Manual ribotyping and the RiboPrinter(R) Microbial Characterization System were used. The two genomic DNA groups could be differentiated each by ribotyping. Our study showed that ribotyping could characterize Methylophaga species and identify unknown isolates. All results were confirmed by DNA/DNA hybridization. Methylophaga sulfidovorans species was confirmed to be a third species by ribotyping and DNA/DNA hybridization.
引用
收藏
页码：372 / 377
页数：6
相关论文
共 50 条
  • [31] Short Read Lengths Recover Ecological Patterns in 16S rRNA Gene Amplicon Data
    Jurburg, Stephanie D.
    MOLECULAR ECOLOGY RESOURCES, 2025,
  • [32] Use of rpoB gene analysis for identification of nitrogen-fixing Paenibacillus species as an alternative to the 16S rRNA gene
    da Mota, FF
    Gomes, EA
    Paiva, E
    Rosado, AS
    Seldin, L
    LETTERS IN APPLIED MICROBIOLOGY, 2004, 39 (01) : 34 - 40
  • [33] RESTRICTION FRAGMENT PATTERNS AS MOLECULAR MARKERS FOR SPECIES IDENTIFICATION AND PHYLOGENETIC ANALYSIS IN THE GENUS ENCHYTRAEUS (OLIGOCHAETA)
    SCHLEGEL, M
    STEINBRUCK, G
    KRAMER, M
    BROCKMEYER, V
    ZEITSCHRIFT FUR ZOOLOGISCHE SYSTEMATIK UND EVOLUTIONSFORSCHUNG, 1991, 29 (5-6): : 362 - 372
  • [34] Molecular characterization of Fusarium oxysporum by PCR amplification of 18S rRNA gene region
    Kuppusamy, Amutha
    Amar, Godavari
    Roshni, Nedumpurath Pallikkara
    INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY, 2018, 56 (11) : 842 - 846
  • [35] Sequence and polymerase chain reaction-restriction fragment length polymorphism analysis of the large subunit rRNA gene of bivalve: Simple and widely applicable technique for multiple species identification of bivalve larva
    Hosoi, M
    Hosoi-Tanabe, S
    Sawada, H
    Ueno, M
    Toyohara, H
    Hayashi, I
    FISHERIES SCIENCE, 2004, 70 (04) : 629 - 637
  • [36] Universal primers to amplify the complete mitochondrial 12S rRNA gene in marine fish species
    Jin, X. X.
    Zhao, S. L.
    Wang, R. X.
    GENETICS AND MOLECULAR RESEARCH, 2013, 12 (04): : 4575 - 4578
  • [37] The evaluation of an identification algorithm for Mycobacterium species using the 16S rRNA coding gene and rpoB
    Kazumi, Yuko
    Mitarai, Satoshi
    INTERNATIONAL JOURNAL OF MYCOBACTERIOLOGY, 2012, 1 (01) : 21 - 28
  • [38] Two new species of Ripella (Amoebozoa, Vannellida) and unusual intragenomic variability in the SSU rRNA gene of this genus
    Kudryavtsev, Alexander
    Gladkikh, Anna
    EUROPEAN JOURNAL OF PROTISTOLOGY, 2017, 61 : 92 - 106
  • [39] Phylogenetic relationships between Vorticella convallaria and other species inferred from small subunit rRNA gene sequences
    Itabashi, T
    Mikami, K
    Fang, J
    Asai, H
    ZOOLOGICAL SCIENCE, 2002, 19 (08) : 931 - 937
  • [40] Identification of Vietnamese Coptotermes pest species based on the sequencing of two regions of 16S rRNA gene
    Thi-Thao Nguyen
    Thi-Huyen Do
    Thu-Huong Duong
    Quynh-Giang Le
    Trong-Khoa Dao
    Thi-Trung Nguyen
    Thi-Quy Nguyen
    Thi-Thu-Hien Nguyen
    Kimura, Keitarou
    Nam-Hai Truong
    BULLETIN OF INSECTOLOGY, 2014, 67 (01): : 131 - 136
12345