Characterization of Methylophaga species by rRNA gene restriction patterns (ribotyping)

被引：3
|
作者：
Janvier, M [1 ]
Grimont, RAD [1 ]
Grimont, F [1 ]
机构：
[1] Inst Pasteur, INSERM, U389, Unite Enterobacteries, F-75724 Paris 15, France
来源：
SYSTEMATIC AND APPLIED MICROBIOLOGY | 1999年 / 22卷 / 03期
关键词：
Methylophaga; ribotyping; taxonomy; identification; environment;
D O I：
10.1016/S0723-2020(99)80045-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A total of 24 strains of Methylophaga, marine methylotrophic bacteria, were characterized by rRNA gene restriction patterns (ribotypes), after cleavage of total DNA with EcoRI or HindIII restriction endonuclease. Manual ribotyping and the RiboPrinter(R) Microbial Characterization System were used. The two genomic DNA groups could be differentiated each by ribotyping. Our study showed that ribotyping could characterize Methylophaga species and identify unknown isolates. All results were confirmed by DNA/DNA hybridization. Methylophaga sulfidovorans species was confirmed to be a third species by ribotyping and DNA/DNA hybridization.
引用
收藏
页码：372 / 377
页数：6
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