Selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities

被引：105

作者：

Toledo, FL ^{[1
]}

Calvo, C ^{[1
]}

Rodelas, B ^{[1
]}

González-López, J ^{[1
]}

机构：

[1] Univ Granada, Inst Water Res, Dept Microbiol, Grp Environm Microbiol, E-18071 Granada, Spain

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2006年 / 29卷 / 03期

关键词：

Bacillus; Alcaligenes; micrococcus; Enterobacter; hydrocarbon; PAH; bioemulsifier;

D O I：

10.1016/j.syapm.2005.09.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fifteen bacterial strains isolated from solid waste oil samples were selected due to their capacity of growing in the presence of hydrocarbons. The isolates were identified by PCR of the 16S rDNA gene using fD1 and rD1 primers. The majority of the strains belonged to genera Bacillus, Bacillus pumilus (eight strains) and Bacillus subtilis (two strains). Besides, three strains were identified as Micrococcus luteus, one as Alcaligenes faecalis and one strain as Enterobacter sp. Growth of the above-mentioned strains in mineral liquid media amended with naphthalene, phenanthrene, fluoranthene or pyrene as sole carbon source was studied and our results showed that these strains can tolerate and remove different polycyclic aromatic hydrocarbons that may be toxic in the environment polluted with hydrocarbons. Finally, the capacity of certain strains to emulsify octane, xilene, toluene, mineral oil and crude oil, and its ability to remove hydrocarbons, look promising for its application in bioremediation technologies. (c) 2005 Elsevier GmbH. All rights reserved.

引用

页码：244 / 252

页数：9

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