Application of the Dual-Luciferase Reporter Assay to the Analysis of Mouse Kit and Yy1 Promoter Activation Regulation by Sohlh1

Kit is significantly downregulated in Sohlh1 ovaries and Sohlh1 was over-expressed in Yy1 cKO ovaries and whether Sohlh1 regulates kit and Yy1 transcription is unknown. Here, researchers examine the promoter transcription activation of Kit and Yy1 regulated by Sohlh1 using dual-luciferase reporter assays. The Kit responsiveness of the Sohlh1 gene has been validated and provided evidence of Sohlh1 transcriptional activation of the Kit gene promoter in the mouse ovary. In contrast, Sohlh1 has little effect on the essential activity of Yy1 promoter. Researchers identifid -1769 to -297 as the transcriptional activitation region of Yy1 promoter and revealed strong inhibitor in -297 to +135 region where maybe G-quadruplexes motifs exist. Researchers found two Yy1 binding Sites in Sohlh1 promoter and surmise that Yy1 negatively regulates Sohlh1 in mouse ovary.