Synthesis of hydroxyethylcellulose-g-methoxypoly (ethylene glycol) copolymer and its application for protein separation in CE

被引：6

作者：

Shi, Xiaohua ^{[1
]}

Tan, Lin ^{[1
]}

Xing, Jinxing ^{[1
]}

Cao, Fuhu ^{[1
]}

Chen, Lijuan ^{[1
]}

Luo, Zhaofeng ^{[2
]}

Wang, Yanmei ^{[1
]}

机构：

[1] Univ Sci & Technol China, Dept Polymer Sci & Engn, CAS Key Lab Soft Matter Chem, Hefei 230026, Peoples R China

[2] Univ Sci & Technol China, Instrument Ctr Life Sci, Hefei 230027, Peoples R China

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 2013年 / 128卷 / 03期

基金：

中国国家自然科学基金;

关键词：

cellulose and other wood products; coatings; copolymers; grafting; proteins; CAPILLARY-ELECTROPHORESIS; N-ISOPROPYLACRYLAMIDE; RAFT POLYMERIZATION; CELLULOSE; PERVAPORATION; MEMBRANES; COATINGS; FILM; DNA;

D O I：

10.1002/app.38403

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Hydroxyethylcellulose-g-methoxypoly (ethylene glycol) (HEC-g-PEG) graft copolymers were synthesized through the etherification reaction between the hydroxyl group of hydroxyethylcellulose (HEC) and iodinated methoxypoly (ethylene glycol) (PEG-I), which was prepared on the basis of two-step reaction. Fourier transforms infrared spectrum (FTIR), nuclear magnetic resonance (NMR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), and iodide oxidation method were used to prove the success of synthesis of graft copolymer. Furthermore, the comparative studies of electro-osmotic flow (EOF) and protein separation in bare-fused silica, HEC and HEC-g-PEG-coated capillary were performed in capillary electrophoresis (CE). The results showed that HEC-g-PEG-coated capillary presented efficient EOF suppression ability and excellent resisting protein adsorption ability. (c) 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013

引用

页码：1995 / 2002

页数：8

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