IL-27 suppresses airway inflammation, hyperresponsiveness and remodeling via the STAT1 and STAT3 pathways in mice with allergic asthma

被引:26
|
作者
Lu, Degan [1 ]
Lu, Jiameng [2 ]
Ji, Xiaoqing [3 ]
Ji, Yanbo [1 ]
Zhang, Zewen [4 ]
Peng, Haiying [5 ]
Sun, Fei [5 ]
Zhang, Caiqing [1 ]
机构
[1] Shandong Univ, Dept Resp Med & Crit Care, Shandong Qianfoshan Hosp, Cheeloo Coll Med, 16766 Jingshi Rd, Jinan 250014, Shandong, Peoples R China
[2] Jilin Med Coll, Dept Biomed Engn, Jilin 132013, Jilin, Peoples R China
[3] Shandong Univ, Div Disinfectant & Supply, Shandong Qianfoshan Hosp, Cheeloo Coll Med, Jinan 250014, Shandong, Peoples R China
[4] Shandong Univ, Dept Magnet Resonance, Shandong Med Imaging Res Inst, Jinan 250021, Shandong, Peoples R China
[5] Shan Dong First Med Univ, Fac Grad, Jinan 271016, Shandong, Peoples R China
关键词
asthma; interleukin; 27; signal transducer and activator of transcription 1; signal transducer and activator of transcription 3; CD4(+) T-CELLS; MURINE MODEL; PULMONARY RESPONSES; SIGNAL TRANSDUCER; GENE-EXPRESSION; TH2; RESPONSES; CUTTING EDGE; DIFFERENTIATION; BET; INTERLEUKIN-27;
D O I
10.3892/ijmm.2020.4622
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Type 2 cytokine-associated immunity may be involved in the pathogenesis of allergic asthma. Although interleukin 27 (IL-27) has been reported as an initiator and suppressor of T-helper 1 (Th1) and T-helper 2 (Th2) responses, respectively, its effects on the development of asthma remain unclear. In the present study, mice were induced and challenged with ovalbumin and received subsequent intranasal administration of IL-27. Total and differential cell counts were determined from Wright-Giemsa-stained cytospins, whereas the cytokine levels were detected using ELISA. In addition, the expression levels of signal transducer and activator of transcription (STAT) 1, STAT3, GATA-binding protein-3 (GATA3) and T-bet (T-box transcription factor) were analyzed in T cells by western blot analysis. Their corresponding mRNA expression levels were determined by quantitative PCR. Airway remodeling was assessed by conventional pathological techniques. The results indicated that intranasal administration of IL-27 ameliorated airway inflammation and hyperresponsiveness in an acute model of asthma. Furthermore, IL-27 prevented airway remodeling in a chronic model of asthma. Following administration of IL-27, the mRNA expression levels of STAT1 and T-bet were upregulated, while those of GATA3 were downregulated. Moreover, the phosphorylation levels of STAT1 and STAT3 were increased. Taken together, these findings demonstrated that intranasal administration of IL-27 ameliorated Th2-related allergic lung inflammation and remodeling in mouse models of asthma by repairing both the STAT1 and STAT3 pathways.
引用
收藏
页码:641 / 652
页数:12
相关论文
共 50 条
  • [31] Characterization of the roles of STAT1 and STAT3 signal transduction pathways in mammalian lens development
    Ebong, S
    Chepelinsky, AB
    Robinson, ML
    Zhao, HT
    Yu, CR
    Egwuagu, CE
    MOLECULAR VISION, 2004, 10 (15-16): : 122 - 131
  • [32] IL-27 controls allergic airway inflammation via Foxp3+regulatory T cells
    Quang Tam Nguyen
    Min, Booki
    JOURNAL OF IMMUNOLOGY, 2018, 200 (01):
  • [33] lncRNA NORAD modulates STAT3/STAT1 balance and innate immune responses in human cells via interaction with STAT3
    Argoetti, Amir
    Shalev, Dor
    Polyak, Galia
    Shima, Noa
    Biran, Hadas
    Lahav, Tamar
    Hashimshony, Tamar
    Mandel-Gutfreund, Yael
    NATURE COMMUNICATIONS, 2025, 16 (01)
  • [34] IL-27 regulates autophagy in rheumatoid arthritis fibroblast-like synoviocytes via STAT3 signaling
    Hou, Jiehong
    Gao, Wei
    IMMUNOBIOLOGY, 2022, 227 (04)
  • [35] Corticosteroid insensitivity persists in the absence of STAT1 signaling in severe allergic airway inflammation
    Lewis, Brandon W.
    Jackson, Devine
    Amici, Stephanie A.
    Walum, Joshua
    Guessas, Manel
    Guessas, Sonia
    Coneglio, Elise
    Boda, Akhila V.
    Guerau-de-Arellano, Mireia
    Grayson, Mitchell H.
    Britt, Rodney D., Jr.
    AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2021, 321 (06) : L1194 - L1205
  • [36] Interleukin-37 alleviates airway inflammation and remodeling in asthma via inhibiting the activation of NF-κB and STAT3 signalings
    Huang, Nina
    Liu, Kebei
    Liu, Jianping
    Gao, Xiaopeng
    Zeng, Zhu
    Zhang, Yudan
    Chen, Jing
    INTERNATIONAL IMMUNOPHARMACOLOGY, 2018, 55 : 198 - 204
  • [37] STAT3 and STAT1 mediate IL-11-dependent and inflammation-associated gastric tumorigenesis in gp130 receptor mutant mice
    Ernst, Matthias
    Najdovska, Meri
    Grail, Dianne
    Lundgren-May, Therese
    Buchert, Michael
    Tye, Hazel
    Matthews, Vance B.
    Armes, Jane
    Bhathal, Prithi S.
    Hughes, Norman R.
    Marcusson, Eric G.
    Karras, James G.
    Na, Songqing
    Sedgwick, Jonathon D.
    Hertzog, Paul J.
    Jenkins, Brendan J.
    JOURNAL OF CLINICAL INVESTIGATION, 2008, 118 (05): : 1727 - 1738
  • [38] IL-27 underpins steroid resistant airway hyperresponsiveness via MyD88 dependent pathways
    Foster, P.
    Li, J.
    Wang, W.
    Baines, K.
    Bowden, N.
    Hansbro, P.
    Gibson, P.
    Kumar, R.
    Yang, M.
    ALLERGY, 2011, 66 : 442 - 443
  • [39] TNFα suppressed IL-33 expression induced by STAT1 and STAT3 dependent signal
    Tsuda, H.
    Komine, M.
    Ohtsuki, M.
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2018, 138 (05) : S132 - S132
  • [40] Differential expression of iL10r2, STAT1 and STAT3 in colorectal cancer
    Movadat, Oliver
    Gruber, Sabine G.
    Agu, Chukwuma A.
    Marian, Brigitte
    Wrba, Friedrich
    Gasche, Christoph
    GASTROENTEROLOGY, 2008, 134 (04) : A384 - A384