Crystallization and preliminary X-ray crystallographic studies of the N-terminal domain of FadD28, a fatty-acyl AMP ligase from Mycobacterium tuberculosis

被引：13

作者：

Goyal, A

Yousuf, M

Rajakumara, E

Arora, P

Gokhale, RS

Sankaranarayanan, R

机构：

[1] Ctr Cellular & Mol Biol, Hyderabad 500007, Andhra Pradesh, India

[2] Natl Inst Immunol, New Delhi 110067, India

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2006年 / 62卷

基金：

英国惠康基金;

关键词：

D O I：

10.1107/S1744309106005938

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

FadD28 from Mycobacterium tuberculosis belongs to the fatty-acyl AMP ligase (FAAL) family of proteins. It is essential for the biosynthesis of a virulent phthiocerol dimycocerosate (PDIM) lipid that is only found in the cell wall of pathogenic mycobacteria. The N-terminal domain, comprising of the first 460 residues, was crystallized by the hanging-drop vapour-diffusion method at 295 K. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.97, b = 60.74, c = 136.54 angstrom. The crystal structure of the N-terminal domain of FadD28 at 2.35 angstrom resolution has been solved using the MAD method.

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收藏

页码：350 / 352

页数：3

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