Osteogenic activity and antibacterial effect of zinc oxide/carboxylated graphene oxide nanocomposites: Preparation and in vitro evaluation

被引：59

作者：

Chen, Junyu ^{[1
,2
]}

Zhang, Xin ^{[1
,2
]}

Cai, He ^{[1
,2
]}

Chen, Zhiqiang ^{[1
,2
]}

Wang, Tong ^{[1
,2
]}

Jia, Lingling ^{[1
,2
]}

Wang, Jian ^{[1
,2
]}

Wan, Qianbing ^{[1
,2
]}

Pei, Xibo ^{[1
,2
]}

机构：

[1] Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu 610041, Peoples R China

[2] Sichuan Univ, West China Hosp Stomatol, Dept Prosthodont, 14,Sect 3,South Peoples Rd, Chengdu 610041, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2016年 / 147卷

关键词：

Zinc oxide; Carboxylated graphene oxide; Nanocomposites; Osteogenic activity; Antibacterial effect; SURFACE-ROUGHNESS; STEM-CELLS; DIFFERENTIATION; NANOPARTICLES; TRANSPORTER; PROTEINS; ION; COMPOSITES; EXPRESSION; PHOSPHATE;

D O I：

10.1016/j.colsurfb.2016.08.023

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The aim of this study was to prepare nanocomposites of carboxylated graphene oxide (GO-COOH) sheets decorated with zinc oxide (ZnO) nanoparticles (NPs) and investigate their advantages in the field of bone tissue engineering. First, ZnO/GO-COOH nanocomposites were synthesized by facile reactions, including the carboxylation of graphene oxide (GO) and the nucleation of ZnO on GO-COOH sheets. The synthesized ZnO/GO-COOH nanocomposites were then characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Raman spectra, and transmission electron microscopy (TEM). The biocompatibility, osteogenic activity and antibacterial effect of ZnO/GO-COOH nanocomposites were further investigated. In the nanocomposites, ZnO nanoparticles with a size of approximately 12 nm were uniformly decorated on GO-COOH sheets. Compared with GO-COOH and the control group, ZnO/GO-COOH nanocomposites significantly enhanced ALP activity, osteocalcin production and extracellular matrix mineralization as well as up-regulated osteogenic-related genes (ALP, OCN, and Runx2) in MG63 osteoblast-like cells. Moreover, ZnO/GO-COOH nanocomposites had an antibacterial effect against Streptococcus mutans. These results indicated that ZnO/GO-COOH nanocomposites exhibited both osteogenic activity and antibacterial effect and had great potential for designing new biomaterials in the field of bone tissue engineering. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：397 / 407

页数：11

共 60 条

[1] Carboxyl-modified single-wall carbon nanotubes improve bone tissue formation in vitro and repair in an in vivo rat model [J].

Barrientos-Duran, Antonio ;

Carpenter, Ellen M. ;

zur Nieden, Nicole I. ;

Malinin, Theodore I. ;

Carlos Rodriguez-Manzaneque, Juan ;

Zanello, Laura P. .

INTERNATIONAL JOURNAL OF NANOMEDICINE, 2014, 9 :4277-4291

[2] A Facile One-step Method to Produce Graphene-CdS Quantum Dot Nanocomposites as Promising Optoelectronic Materials [J].

Cao, Aoneng ;

Liu, Zhen ;

Chu, Saisai ;

Wu, Minghong ;

Ye, Zhangmei ;

Cai, Zhengwei ;

Chang, Yanli ;

Wang, Shufeng ;

Gong, Qihuang ;

Liu, Yuanfang .

ADVANCED MATERIALS, 2010, 22 (01) :103-+

[3] In vitro toxicity evaluation of graphene oxide on A549 cells [J].

Chang, Yanli ;

Yang, Sheng-Tao ;

Liu, Jia-Hui ;

Dong, Erya ;

Wang, Yanwen ;

Cao, Aoneng ;

Liu, Yuanfang ;

Wang, Haifang .

TOXICOLOGY LETTERS, 2011, 200 (03) :201-210

[4] Synthesis of silver nanoparticles in an aqueous suspension of graphene oxide sheets and its antimicrobial activity [J].

Das, Manash R. ;

Sarma, Rupak K. ;

Saikia, Ratul ;

Kale, Vinayak S. ;

Shelke, Manjusha V. ;

Sengupta, Pinaki .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2011, 83 (01) :16-22

[5] Antimicrobial Electrospun Biopolymer Nanofiber Mats Functionalized with Graphene Oxide-Silver Nanocomposites [J].

de Faria, Andreia F. ;

Perreault, Francois ;

Shaulsky, Evyatar ;

Chavez, Laura H. Arias ;

Elimelech, Menachem .

ACS APPLIED MATERIALS & INTERFACES, 2015, 7 (23) :12751-12759

[6] Anti-adhesion and antibacterial activity of silver nanoparticles supported on graphene oxide sheets [J].

de Faria, Andreia Fonseca ;

Teodoro Martinez, Diego Stefani ;

Meister Meira, Stela Mans ;

Mazarin de Moraes, Ana Carolina ;

Brandelli, Adriano ;

Souza Filho, Antonio Gomes ;

Alves, Oswaldo Luiz .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2014, 113 :115-124

[7] Functionalized Graphene Oxide as a Nanocarrier in a Multienzyme Labeling Amplification Strategy for Ultrasensitive Electrochemical Immunoassay of Phosphorylated p53 (S392) [J].

Du, Dan ;

Wang, Limin ;

Shao, Yuyan ;

Wang, Jun ;

Engelhard, Mark H. ;

Lin, Yuehe .

ANALYTICAL CHEMISTRY, 2011, 83 (03) :746-752

[8] Differential regulation of osteogenic differentiation of stem cells on surface roughness gradients [J].

Faia-Torres, Ana B. ;

Guimond-Lischer, Stefanie ;

Rottmar, Markus ;

Charnley, Mirren ;

Goren, Tolga ;

Maniura-Weber, Katharina ;

Spencer, Nicholas D. ;

Reis, Rui L. ;

Textor, Marcus ;

Neves, Nuno M. .

BIOMATERIALS, 2014, 35 (33) :9023-9032

[9]

Farrusseng D, 2011, METAL-ORGANIC FRAMEWORKS: APPLICATIONS FROM CATALYSIS TO GAS STORAGE, P1, DOI 10.1002/9783527635856

[10] SiO2 and ZnO dopants in three-dimensionally printed tricalcium phosphate bone tissue engineering scaffolds enhance osteogenesis and angiogenesis in vivo [J].

Fielding, Gary ;

Bose, Susmita .

ACTA BIOMATERIALIA, 2013, 9 (11) :9137-9148

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