Pseudomonas aeruginosa Type-3 Secretion System Dampens Host Defense by Exploiting the NLRC4-coupled Inflammasome

被引：71

作者：

Faure, Emmanuel ^{[1
,2
]}

Mear, Jean-Baptiste ^{[1
,2
]}

Faure, Karine ^{[1
,2
]}

Normand, Sylvain ^{[2
,3
,4
,5
]}

Couturier-Maillard, Aurelie ^{[2
,3
,4
,5
]}

Grandjean, Teddy ^{[2
,3
,4
,5
]}

Balloy, Viviane ^{[6
]}

Ryffel, Bernhard ^{[7
,8
]}

Dessein, Rodrigue ^{[1
,2
]}

Chignard, Michel ^{[6
]}

Uyttenhove, Catherine ^{[7
,8
]}

Guery, Benoit ^{[1
,2
]}

Gosset, Philippe ^{[2
,3
,4
,5
]}

Chamaillard, Mathias ^{[2
,3
,4
,5
]}

Kipnis, Eric ^{[1
,2
]}

机构：

[1] Univ Droit & Sante Lille, Pseudomonas Aeruginosa Host Pathogen Translat Res, Lille, France

[2] Univ Lille Nord France, Lille, France

[3] Ctr Infect & Immun Lille, Inst Pasteur Lille, Lille, France

[4] Inst Pasteur, Ctr Immunol & Biol Parasitaire, CNRS, UMR 8204, F-59019 Lille, France

[5] INSERM, U1019, F-59045 Lille, France

[6] Inst Pasteur, Unite Def Innee & Inflammat, Paris, France

[7] UMR 7355, Orleans, France

[8] Univ Cape Town, Inst Infect Dis & Mol Med, ZA-7925 Cape Town, South Africa

来源：

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE | 2014年 / 189卷 / 07期

关键词：

Pseudomonas aeruginosa; type-3 secretion system; inflammasome; antimicrobial peptides; ACUTE LUNG INJURY; NLRC4; INFLAMMASOME; V-ANTIGEN; PNEUMONIA; INFECTION; PROTEIN; MECHANISMS; IMMUNITY; CELLS; PHOSPHORYLATION;

D O I：

10.1164/rccm.201307-1358OC

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

Rationale: Pseudomonas aeruginosa, a major problem pathogen responsible for severe infections in critically ill patients, triggers, through a functional type-3 secretion system (T3SS), the activation of an intracellular cytosolic sensor of innate immunity, NLRC4. Although the NLRC4-inflammasome-dependent response contributes to increased clearance of intracellular pathogens, it seems that NLRC4 inflammasome activation decreases the clearance of P. aeruginosa, a mainly extracellular pathogen. Objectives: We sought to determine the underlying mechanisms of this effect of the activation of NLRC4 by P. aeruginosa. Methods: We established acute lung injury in wild-type and Nlrc4(-/-) mice using sublethal intranasal inocula of P. aeruginosa strain CHA expressing or not a functional T3SS. We studied 96-hour survival, lung injury, bacterial clearance from the lungs, cytokine secretion in bronchoalveolar lavage, lung antimicrobial peptide expression by quantitative polymerase chain reaction, and flow cytometry analysis of lung cells. Measurements and Main Results: Nlrc4(-/-) mice showed enhanced bacterial clearance and decreased lung injury contributing to increased survival against extracellular P. aeruginosa strain expressing a functional T3SS. The mechanism involved decreased NLRC4-inflammasome- driven IL-18 secretion attenuating lung injury caused by excessive neutrophil recruitment. Additionally, in the lungs of Nlrc4(-/-) mice secretion of IL-17 by innate immune cells was increased and responsible for increased expression of lung epithelial antimicrobial peptides. Furthermore, IL-18 secretion was found to repress IL-17 and IL-17-driven lung antimicrobial peptide expression. Conclusions: We report a new role of the T3SS apparatus itself, independently of exotoxin translocation. Through NLRC4 inflammasome activation, the T3SS promotes IL-18 secretion, which dampens a beneficial IL-17-mediated antimicrobial host response.

引用

页码：799 / 811

页数：13

共 46 条

[1] Alveolar response to Pseudomonas aeruginosa:: Role of the type III secretion system [J].