Dynamic high pressure microfluidization-assisted extraction and bioactivities of Cyperus esculentus (C. esculentus L.) leaves flavonoids

The aim of this work was to study the effect of dynamic high pressure microfluidization (DHPM) on extracting total flavonoids from Cyperus esculentus L. (C. esculentus L.) leaves and to evaluate the antioxidant activity and antibacterial property of these flavonoids. In all the assays, pretreatment with DHPM was found to not only efficiently improve the yield of total flavonoids but also strengthen the antioxidant activity of the total flavonoids. C. esculentus L. leaves flavonoids had pronounced antioxidant activity in vivo that could significantly elevate the content of superoxide dismutase (SOD) without increasing the malondialdehyde (MDA) levels, and could also improve total antioxidant capacity in mice with a dose-dependent fashion. C. esculentus L. leaves flavonoids inhibited the growth of both Gram positive and Gram negative bacteria while no obvious inhibitory effect on Penicillium and Aspergiltus could be observed. Our studies indicate that flavonoids from C. esculentus L. leaves can be taken as a natural antioxidant and bacteriostatic substance in food and pharmaceutical industry. (C) 2015 Elsevier Ltd. All rights reserved.