REAL-TIME PCR QUANTIFICATION OF PLASMA DNA IN NON-SMALL CELL LUNG CANCER PATIENTS AND HEALTHY CONTROLS

被引:17
作者
Szpechcinski, A. [1 ]
Dancewicz, M. [2 ]
Kopinski, P. [3 ]
Kowalewski, J. [2 ]
Chorostowska-Wynimko, J. [1 ]
机构
[1] Natl Inst TB & Lung Dis, Lab Mol Diagnost & Immunol, Plocka 26 St, PL-01138 Warsaw, Poland
[2] Nicolaus Copernicus Univ, Dept Thorac Surg & Tumors, Ctr Oncol, Bydgoszcz, Poland
[3] Nicolaus Copernicus Univ, Chair Gene Therapy, Collegium Med, Bydgoszcz, Poland
关键词
DNA quantification; free-circulating DNA; lung cancer; plasma; real-time PCR;
D O I
10.1186/2047-783X-14-S4-237
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Introduction: Free-circulating DNA is present in minute amounts in plasma of healthy individuals, whereas increased levels are found in a number of malignant pathologies including non-small cell lung cancer (NSCLC). The objective of this research was the evaluation of the plasma DNA quantification capacity to distinguish between healthy subjects and non-small cell lung cancer (NSCLC) patients. Material and methods: Plasma samples were collected prospectively from 16 healthy volunteers and 30 untreated NSCLC patients (I-IIIA). Subsequently, freecirculating DNA extraction and quantitative real-time PCR analysis were performed. Results: The values of plasma DNA concentration ranged from 0.9 up to 7.0 ng/ml in healthy individuals and from 1.5 up to 50 ng/ml in NSCLC patients before treatment. Cancer group showed several-fold higher mean free-circulating DNA concentration than that present in healthy subjects (mean 12.00 vs. 2.65 ng/ml; P < 0.001). A greater variability of plasma DNA concentrations was observed in NSCLC patients than in controls (SD 14.50 vs. 2.02, respectively). The area under the ROC curve was 0.87 (95% CI, 0.744 to 0.954, P < 0.001). Conclusion: Non-small cell lung cancer is associated with elevated levels of cell-free DNA in plasma with respect to healthy controls. Real-time PCR method proved its utility in effective free-circulating DNA detection and quantification.
引用
收藏
页码:237 / 240
页数:4
相关论文
共 50 条
  • [1] Real-time PCR quantification of plasma DNA in non-small cell lung cancer patients and healthy controls
    A Szpechcinski
    M Dancewicz
    P Kopinski
    J Kowalewski
    J Chorostowska-Wynimko
    European Journal of Medical Research, 14
  • [2] Comparative Quantification of Plasma hnRNP B1 mRNA in Non-small Cell Lung Cancer Patients by Real-time PCR
    Kim, Jeong-Man
    Hwang, Sang Hyun
    Song, Eun Ju
    Sang-Yull, Lee
    Kim, Yeong-Dae
    Lee, Chang Hun
    Lee, Min Ki
    Chang, Chulhun L.
    Lee, Eun Yup
    KOREAN JOURNAL OF LABORATORY MEDICINE, 2009, 29 (03): : 249 - 255
  • [3] Fully automated real-time PCR for EGFR testing in non-small cell lung carcinoma
    Richard Colling
    Hollie Bancroft
    Gerald Langman
    Elizabeth Soilleux
    Virchows Archiv, 2019, 474 : 187 - 192
  • [4] Fully automated real-time PCR for EGFR testing in non-small cell lung carcinoma
    Colling, Richard
    Bancroft, Hollie
    Langman, Gerald
    Soilleux, Elizabeth
    VIRCHOWS ARCHIV, 2019, 474 (02) : 187 - 192
  • [5] Quantification of total plasma cell-free DNA in ovarian cancer using real-time PCR
    Kamat, Aparna A.
    Sood, Anil K.
    Dang, Dianne
    Gershenson, David M.
    Simpson, Joe L.
    Bischoff, Farideh Z.
    CIRCULATING NUCLEIC ACIDS IN PLASMA AND SERUM IV, 2006, 1075 : 230 - 234
  • [6] Comparison of plasma to tissue DNA mutations in surgical patients with non-small cell lung cancer
    Chen, Kezhong
    Zhang, Jingbo
    Guan, Tian
    Yang, Fan
    Lou, Feng
    Chen, Wei
    Zhao, Mingyu
    Zhang, Jay
    Chen, Siyi
    Wang, Jun
    JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, 2017, 154 (03) : 1123 - 1130
  • [7] Circulating plasma DNA as diagnostic biomarker in non-small cell lung cancer
    Paci, Massimiliano
    Maramotti, Sally
    Bellesia, Enrica
    Formisano, Debora
    Albertazzi, Laura
    Ricchetti, Tommaso
    Ferrari, Guglielmo
    Annessi, Valerio
    Lasagni, Daniela
    Carbonelli, Cristiano
    De Franco, Salvatore
    Brini, Maria
    Sgarbi, Giorgio
    Lodi, Renzo
    LUNG CANCER, 2009, 64 (01) : 92 - 97
  • [8] Cell-free DNA levels in plasma of patients with non-small-cell lung cancer and inflammatory lung disease
    Szpechcinski, A.
    Chorostowska-Wynimko, J.
    Struniawski, R.
    Kupis, W.
    Rudzinski, P.
    Langfort, R.
    Puscinska, E.
    Bielen, P.
    Sliwinski, P.
    Orlowski, T.
    BRITISH JOURNAL OF CANCER, 2015, 113 (03) : 476 - 483
  • [9] DNA quantification by real-time PCR in different forensic samples
    Martins, C.
    Lima, G.
    Carvalho, M. R.
    Caine, L.
    Porto, M. J.
    FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES, 2015, 5 : E545 - E546
  • [10] Assessment of the real-time PCR and different digital PCR platforms for DNA quantification
    Jernej Pavšič
    Jana Žel
    Mojca Milavec
    Analytical and Bioanalytical Chemistry, 2016, 408 : 107 - 121