DNA quantification;
free-circulating DNA;
lung cancer;
plasma;
real-time PCR;
D O I:
10.1186/2047-783X-14-S4-237
中图分类号:
R-3 [医学研究方法];
R3 [基础医学];
学科分类号:
1001 ;
摘要:
Introduction: Free-circulating DNA is present in minute amounts in plasma of healthy individuals, whereas increased levels are found in a number of malignant pathologies including non-small cell lung cancer (NSCLC). The objective of this research was the evaluation of the plasma DNA quantification capacity to distinguish between healthy subjects and non-small cell lung cancer (NSCLC) patients. Material and methods: Plasma samples were collected prospectively from 16 healthy volunteers and 30 untreated NSCLC patients (I-IIIA). Subsequently, freecirculating DNA extraction and quantitative real-time PCR analysis were performed. Results: The values of plasma DNA concentration ranged from 0.9 up to 7.0 ng/ml in healthy individuals and from 1.5 up to 50 ng/ml in NSCLC patients before treatment. Cancer group showed several-fold higher mean free-circulating DNA concentration than that present in healthy subjects (mean 12.00 vs. 2.65 ng/ml; P < 0.001). A greater variability of plasma DNA concentrations was observed in NSCLC patients than in controls (SD 14.50 vs. 2.02, respectively). The area under the ROC curve was 0.87 (95% CI, 0.744 to 0.954, P < 0.001). Conclusion: Non-small cell lung cancer is associated with elevated levels of cell-free DNA in plasma with respect to healthy controls. Real-time PCR method proved its utility in effective free-circulating DNA detection and quantification.
机构:Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Shatin, Hong Kong, Peoples R China
Lam, NYL
Rainer, TH
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机构:Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Shatin, Hong Kong, Peoples R China
Rainer, TH
Chiu, RWK
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机构:Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Shatin, Hong Kong, Peoples R China
Chiu, RWK
Lo, YMD
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h-index: 0
机构:
Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Shatin, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Shatin, Hong Kong, Peoples R China
机构:
Azienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Paci, Massimiliano
Maramotti, Sally
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h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Dept Clin Pathol, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Maramotti, Sally
Bellesia, Enrica
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h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Dept Clin Pathol, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Bellesia, Enrica
Formisano, Debora
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Stat & Clin Epidemiol Unit, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Formisano, Debora
Albertazzi, Laura
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h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Dept Clin Pathol, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Albertazzi, Laura
Ricchetti, Tommaso
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Ricchetti, Tommaso
Ferrari, Guglielmo
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Ferrari, Guglielmo
Annessi, Valerio
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Annessi, Valerio
Lasagni, Daniela
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Ctr Blood Transfus, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Lasagni, Daniela
Carbonelli, Cristiano
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Div Pulm, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Carbonelli, Cristiano
De Franco, Salvatore
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Innovat & Clin Res Unit, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
De Franco, Salvatore
Brini, Maria
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Dept Clin Pathol, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Brini, Maria
Sgarbi, Giorgio
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Sgarbi, Giorgio
Lodi, Renzo
论文数: 0引用数: 0
h-index: 0
机构:
Univ Modena, Policlin Univ Modena, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
机构:Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Shatin, Hong Kong, Peoples R China
Lam, NYL
Rainer, TH
论文数: 0引用数: 0
h-index: 0
机构:Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Shatin, Hong Kong, Peoples R China
Rainer, TH
Chiu, RWK
论文数: 0引用数: 0
h-index: 0
机构:Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Shatin, Hong Kong, Peoples R China
Chiu, RWK
Lo, YMD
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Shatin, Hong Kong, Peoples R ChinaChinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Shatin, Hong Kong, Peoples R China
机构:
Azienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Paci, Massimiliano
Maramotti, Sally
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Dept Clin Pathol, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Maramotti, Sally
Bellesia, Enrica
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Dept Clin Pathol, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Bellesia, Enrica
Formisano, Debora
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Stat & Clin Epidemiol Unit, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Formisano, Debora
Albertazzi, Laura
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Dept Clin Pathol, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Albertazzi, Laura
Ricchetti, Tommaso
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Ricchetti, Tommaso
Ferrari, Guglielmo
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Ferrari, Guglielmo
Annessi, Valerio
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Annessi, Valerio
Lasagni, Daniela
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Ctr Blood Transfus, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Lasagni, Daniela
Carbonelli, Cristiano
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Div Pulm, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Carbonelli, Cristiano
De Franco, Salvatore
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Innovat & Clin Res Unit, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
De Franco, Salvatore
Brini, Maria
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Dept Clin Pathol, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Brini, Maria
Sgarbi, Giorgio
论文数: 0引用数: 0
h-index: 0
机构:
Azienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy
Sgarbi, Giorgio
Lodi, Renzo
论文数: 0引用数: 0
h-index: 0
机构:
Univ Modena, Policlin Univ Modena, I-42100 Reggio Emilia, ItalyAzienda Osped Santa Maria Nuova, Div Thorac Surg, I-42100 Reggio Emilia, Italy