REAL-TIME PCR QUANTIFICATION OF PLASMA DNA IN NON-SMALL CELL LUNG CANCER PATIENTS AND HEALTHY CONTROLS

被引:17
作者
Szpechcinski, A. [1 ]
Dancewicz, M. [2 ]
Kopinski, P. [3 ]
Kowalewski, J. [2 ]
Chorostowska-Wynimko, J. [1 ]
机构
[1] Natl Inst TB & Lung Dis, Lab Mol Diagnost & Immunol, Plocka 26 St, PL-01138 Warsaw, Poland
[2] Nicolaus Copernicus Univ, Dept Thorac Surg & Tumors, Ctr Oncol, Bydgoszcz, Poland
[3] Nicolaus Copernicus Univ, Chair Gene Therapy, Collegium Med, Bydgoszcz, Poland
关键词
DNA quantification; free-circulating DNA; lung cancer; plasma; real-time PCR;
D O I
10.1186/2047-783X-14-S4-237
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Introduction: Free-circulating DNA is present in minute amounts in plasma of healthy individuals, whereas increased levels are found in a number of malignant pathologies including non-small cell lung cancer (NSCLC). The objective of this research was the evaluation of the plasma DNA quantification capacity to distinguish between healthy subjects and non-small cell lung cancer (NSCLC) patients. Material and methods: Plasma samples were collected prospectively from 16 healthy volunteers and 30 untreated NSCLC patients (I-IIIA). Subsequently, freecirculating DNA extraction and quantitative real-time PCR analysis were performed. Results: The values of plasma DNA concentration ranged from 0.9 up to 7.0 ng/ml in healthy individuals and from 1.5 up to 50 ng/ml in NSCLC patients before treatment. Cancer group showed several-fold higher mean free-circulating DNA concentration than that present in healthy subjects (mean 12.00 vs. 2.65 ng/ml; P < 0.001). A greater variability of plasma DNA concentrations was observed in NSCLC patients than in controls (SD 14.50 vs. 2.02, respectively). The area under the ROC curve was 0.87 (95% CI, 0.744 to 0.954, P < 0.001). Conclusion: Non-small cell lung cancer is associated with elevated levels of cell-free DNA in plasma with respect to healthy controls. Real-time PCR method proved its utility in effective free-circulating DNA detection and quantification.
引用
收藏
页码:237 / 240
页数:4
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