Gut microbiota of obese subjects with Prader-Willi syndrome is linked to metabolic health

被引:36
作者
Olsson, Lisa M. [1 ]
Poitou, Christine [2 ,3 ]
Tremaroli, Valentina [1 ]
Coupaye, Muriel [3 ]
Aron-Wisnewsky, Judith [2 ,3 ]
Backhed, Fredrik [1 ,4 ]
Clement, Karine [2 ,3 ]
Caesar, Robert [1 ]
机构
[1] Univ Gothenburg, Sahlgrenska Acad, Dept Mol & Clin Med, Wallenberg Lab,Inst Med, S-41345 Gothenburg, Sweden
[2] Sorbonne Univ, INSERM, Nutr & Obes Syst Approaches NutriOm Res Unit, Paris, Ile De France, France
[3] Univ Hosp Pitie Salpetriere, AP HP, Reference Ctr Rare Dis Prader Willi Syndrome, Nutr Dept, Paris, Ile De France, France
[4] Univ Copenhagen, Fac Hlth Sci, Sect Metab Receptol & Enteroendocrinol, Novo Nordisk Fdn,Ctr Basic Metab Res, Copenhagen, Denmark
基金
瑞典研究理事会; 欧洲研究理事会;
关键词
intestinal bacteria; diabetes mellitus; glucose metabolism; BODY-COMPOSITION; INSULIN SENSITIVITY; INTESTINAL MICROBIOTA; SYNDROME PWS; ADULTS; RESISTANCE; SIGNATURES; GENE;
D O I
10.1136/gutjnl-2019-319322
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Objective The gut microbiota has been implicated in the aetiology of obesity and associated comorbidities. Patients with Prader-Willi syndrome (PWS) are obese but partly protected against insulin resistance. We hypothesised that the gut microbiota of PWS patients differs from that of non-genetically obese controls and correlate to metabolic health. Therefore, here we used PWS as a model to study the role of gut microbiota in the prevention of metabolic complications linked to obesity. Design We conducted a case-control study with 17 adult PWS patients and 17 obese subjects matched for body fat mass index, gender and age. The subjects were metabolically characterised and faecal microbiota was profiled by 16S ribosomal RNA gene sequencing. The patients' parents were used as a non-obese control group. Stool samples from two PWS patients and two obese controls were used for faecal microbiota transplantations in germ-free mice to examine the impact of the microbiota on glucose metabolism. Results The composition of the faecal microbiota in patients with PWS differed from that of obese controls, and was characterised by higher phylogenetic diversity and increased abundance of several taxa such as Akkermansia, Desulfovibrio and Archaea, and decreased abundance of Dorea. Microbial taxa prevalent in the PWS microbiota were associated with markers of insulin sensitivity. Improved insulin resistance of PWS was partly transmitted by faecal microbiota transplantations into germ-free mice. Conclusion The gut microbiota of PWS patients is similar to that of their non-obese parents and might play a role for the protection of PWS patients from metabolic complications.
引用
收藏
页码:1229 / 1238
页数:10
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