The microbial community of Vetiver root and its involvement into essential oil biogenesis

被引:75
作者
Del Giudice, Luigi [2 ]
Massardo, Domenica Rita [2 ]
Pontieri, Paola [2 ]
Bertea, Cinzia M. [3 ,4 ]
Mombello, Domenico [3 ,4 ]
Carata, Elisabetta [1 ]
Tredici, Salvatore Maurizio [1 ]
Tala, Adelfia [1 ]
Mucciarelli, Marco [5 ]
Groudeva, Veneta Ivanova [6 ]
De Stefano, Mario [7 ]
Vigliotta, Giovanni [1 ]
Maffei, Massimo E. [3 ,4 ]
Alifano, Pietro [1 ]
机构
[1] Univ Salento, Dept Biol & Environm Sci & Technol, I-73100 Lecce, Italy
[2] CNR, Inst Genet & Biophys Adriano Buzzati Traverso, I-80131 Naples, Italy
[3] Univ Turin, Dept Plant Biol, I-10125 Turin, Italy
[4] Univ Salento, Ctr Excellence CEBIOVEM, I-73100 Lecce, Italy
[5] Univ Turin, Dept Vet Morphophysiol, I-10095 Turin, Italy
[6] Univ Sofia, Dept Gen & Ind Microbiol, Sofia 1164, Bulgaria
[7] Univ Naples 2, Dept Environm Sci, I-81100 Caserta, Italy
关键词
D O I
10.1111/j.1462-2920.2008.01703.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Vetiver is the only grass cultivated worldwide for the root essential oil, which is a mixture of sesquiterpene alcohols and hydrocarbons, used extensively in perfumery and cosmetics. Light and transmission electron microscopy demonstrated the presence of bacteria in the cortical parenchymatous essential oil-producing cells and in the lysigen lacunae in close association with the essential oil. This finding and the evidence that axenic Vetiver produces in vitro only trace amounts of oil with a strikingly different composition compared with the oils from in vivo Vetiver plants stimulated the hypothesis of an involvement of these bacteria in the oil metabolism. We used culture-based and culture-independent approaches to analyse the microbial community of the Vetiver root. Results demonstrate a broad phylogenetic spectrum of bacteria, including alpha-, beta- and gamma-Proteobacteria, high-G+C-content Gram-positive bacteria, and microbes belonging to the Fibrobacteres/Acidobacteria group. We isolated root-associated bacteria and showed that most of them are able to grow by using oil sesquiterpenes as a carbon source and to metabolize them releasing into the medium a large number of compounds typically found in commercial Vetiver oils. Several bacteria were also able to induce gene expression of a Vetiver sesquiterpene synthase. These results support the intriguing hypothesis that bacteria may have a role in essential oil biosynthesis opening the possibility to use them to manoeuvre the Vetiver oil molecular structure.
引用
收藏
页码:2824 / 2841
页数:18
相关论文
共 69 条
[1]   Pseudomonas brassicacearum sp nov and Pseudomonas thivervalensis sp nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis thaliana [J].
Achouak, W ;
Sutra, L ;
Heulin, T ;
Meyer, JM ;
Fromin, N ;
Degraeve, S ;
Christen, R ;
Gardan, L .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :9-18
[2]   Preliminary comparison of vetiver root essential oils from cleansed (bacteria- and fungus-free) versus non-cleansed (normal) vetiver plants [J].
Adams, RP ;
Habte, M ;
Park, S ;
Dafforn, MR .
BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2004, 32 (12) :1137-1144
[3]   The use of vetiver for remediation of heavy metal soil contamination [J].
Antiochia, Riccarda ;
Campanella, Luigi ;
Ghezzi, Paola ;
Movassaghi, K. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 388 (04) :947-956
[4]   Ability of bacterium Bacillus subtilis to produce cytokinins and to influence the growth and endogenous hormone content of lettuce plants [J].
Arkhipova, TN ;
Veselov, SU ;
Melentiev, AI ;
Martynenko, EV ;
Kudoyarova, GR .
PLANT AND SOIL, 2005, 272 (1-2) :201-209
[5]   Endophytic and ectophytic potato-associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi [J].
Berg, G ;
Krechel, A ;
Ditz, M ;
Sikora, RA ;
Ulrich, A ;
Hallmann, J .
FEMS MICROBIOLOGY ECOLOGY, 2005, 51 (02) :215-229
[6]   Plant-dependent genotypic and phenotypic diversity of antagonistic rhizobacteria isolated from different Verticillium host plants [J].
Berg, G ;
Roskot, N ;
Steidle, A ;
Eberl, L ;
Zock, A ;
Smalla, K .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (07) :3328-3338
[7]   Rhizosphere communication of plants, parasitic plants and AM fungi [J].
Bouwmeester, Harro J. ;
Roux, Christophe ;
Lopez-Raez, Juan Antonio ;
Becard, Guillaume .
TRENDS IN PLANT SCIENCE, 2007, 12 (05) :224-230
[8]   Potential of vetiver (Vetiveria zizanioides (L.) Nash) for phytoremediation of petroleum hydrocarbon-contaminated soils in Venezuela [J].
Brandt, Regine ;
Merkl, Nicole ;
Schultze-Kraft, Rainer ;
Infante, Carmen ;
Broll, Gabriele .
INTERNATIONAL JOURNAL OF PHYTOREMEDIATION, 2006, 8 (04) :273-284
[9]  
BUCHANAN RE, 1974, BERGEY MANUAL DETERM
[10]   A study on the composition of commercial Vetiveria zizanioides oils from different geographical origins [J].
Champagnat, Pascal ;
Figueredo, Gilles ;
Chalchat, Jean-Claude ;
Carnat, Andre-Paul ;
Bessiere, Jean-Marie .
JOURNAL OF ESSENTIAL OIL RESEARCH, 2006, 18 (04) :416-422