Transcriptional regulation analysis of FAM3A gene and its effect on adipocyte differentiation

被引：6

作者：

Kang, Tingting ^{[1
,2
]}

Peng, Disha ^{[1
,2
]}

Bu, Guowei ^{[1
,2
]}

Gu, Hao ^{[1
,2
]}

Zhang, Feng ^{[1
,3
]}

Zhang, Ruirui ^{[1
,2
]}

Zhou, Ying ^{[1
,2
]}

Xiong, Yuanzhu ^{[1
,2
]}

Lei, Minggang ^{[1
,2
]}

机构：

[1] Huazhong Agr Univ, Coll Anim Sci & Technol, Key Lab Swine Genet & Breeding, Minist Agr, Wuhan 430070, Peoples R China

[2] Huazhong Agr Univ, Coll Anim Sci & Technol, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China

[3] Hubei Acad Agr Sci, Inst Anim Husb & Vet, Hubei Key Lab Anim Embryo Engn & Mol Breeding, Wuhan 430064, Peoples R China

来源：

GENE | 2016年 / 595卷 / 01期

关键词：

FAM3A; Adipogenesis; Promoter; C/EBP beta; ACTIVATED RECEPTOR-GAMMA; PPAR-GAMMA; C/EBP-BETA; STEM-CELL; ADIPOGENESIS; PATHWAY; BINDING; CHROMOSOME; EXPRESSION; SWITCH;

D O I：

10.1016/j.gene.2016.09.038

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

FAM3A (family with sequence similarity 3, member A) is regulated by PPARG and participates in the metabolism of lipid in liver. However, the transcriptional regulation analysis of FAM3A is very little and biological function of FAM3A still unclear. In this study, the core promoter region and transcription factor binding sites of FAM3A gene were identified and characterized using dual luciferase report experiments and electrophoretic mobility shift assays (EMSA). The promoter activity of FAM3A was dramatically decreased after the mutation of C/EBP beta binding sites, suggesting that C/EBP beta is a transcriptional activator of FAM3A. Overexpression of FAM3A significantly inhibited the efficiency of preadipocytes to differentiate into adipocytes as indicated by Western Blot and Oil Red 0 staining assay. These results suggest that C/EBP beta plays an important role in regulating FAM3A promoter activity and FAM3A inhibits adipocyte differentiation. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：92 / 98

页数：7

共 33 条

[1]

ASO T, 1994, J BIOL CHEM, V269, P26575

[2] TRANSCRIPTIONAL ORGANIZATION OF A 450-KB REGION OF THE HUMAN X-CHROMOSOME IN XQ28 [J].