Physiological and enzymatic comparison between Pichia stipitis and recombinant Saccharomyces cerevisiae on xylose fermentation

被引：5

作者：

Guo, Changying ^{[1
]}

Jiang, Ning ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, Beijing 100080, Peoples R China

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2013年 / 29卷 / 03期

关键词：

Saccharomyces cerevisiae; Pichia stipitis; Xylose; Ethanol; Enzymatic analysis; PRODUCT FORMATION; ETHANOL-PRODUCTION; NADPH; YEAST; EXPRESSION; REDUCTASE; CONSUMPTION; IMPROVES; XYL1; XKS1;

D O I：

10.1007/s11274-012-1208-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In order to better understand the differences in xylose metabolism between natural xylose-utilizing Pichia stipitis and metabolically engineered Saccharomyces cerevisiae, we constructed a series of recombinant S. cerevisiae strains with different xylose reductase/xylitol dehydrogenase/xylulokinase activity ratios by integrating xylitol dehydrogenase gene (XYL2) into the chromosome with variable copies and heterogeneously expressing xylose reductase gene (XYL1) and endogenous xylulokinase gene (XKS1). The strain with the highest specific xylose uptake rate and ethanol productivity on pure xylose fermentation was selected to compare to P. stipitis under oxygen-limited condition. Physiological and enzymatic comparison showed that they have different patterns of xylose metabolism and NADPH generation.

引用

页码：541 / 547

页数：7

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