Fluorescent sensor responsive to local viscosity and its application to the imaging of liquid-ordered domain in lipid membranes

被引：23
|
作者：
Yasuhara, Kazuma [1 ]
Sasaki, Yoshihiro [1 ]
Kikuchi, Jun-ichi [1 ]
机构：
[1] Nara Inst Sci & Technol, Grad Sch Mat Sci, Nara 6300192, Japan
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2008年 / 67卷 / 01期
基金：
日本学术振兴会;
关键词：
Liquid-ordered phase; Lipid raft; Fluorescence; Auramine; Giant vesicle;
D O I：
10.1016/j.colsurfb.2008.08.004
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A novel fluorescent sensor responsive to local viscosity in the lipid bilayer membranes was designed and synthesized. The sensor changes its fluorescence intensity reflecting the local viscosity of its surrounding medium. The fluorescence measurement showed that the sensor is capable of discriminating between different phase states of lipid bilayer. In addition, the sensor visualized liquid-ordered microdomains on giant vesicles in terms of the microviscosity with a simple fluorescence technique. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：145 / 149
页数：5
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