Electrophoretic separation of linear and supercoiled DNA in uncoated capillaries

被引：11

作者：

de Carmejane, O ^{[1
]}

Schwinefus, JJ ^{[1
]}

Wang, SC ^{[1
]}

Morris, MD ^{[1
]}

机构：

[1] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 849卷 / 01期

关键词：

DNA; electrophoretic separation; uncoated capillaries;

D O I：

10.1016/S0021-9673(99)00527-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report electrophoretic separation of supercoiled plasmids (2-16 kilo base pairs) and linear double-stranded DNA (0.6-23 kilo base pairs) in uncoated capillaries filled with dilute hydroxyethylcellulose. Because electroosmotic flow reverses the order of elution, long plasmids spend less time in the capillary and their bandwidths are narrower than observed in coated capillaries. However, resolution is similar to that obtained in coated capillaries, because it is governed by the distribution of unresolved topoisomers. In the presence of electroosmotic how migration of supercoiled plasmids does not follow the elastic rod model that has been observed in coated capillaries. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：267 / 276

页数：10

共 50 条

[41] Electrophoretic μPAD for Purification and Analysis of DNA Samples
Heinsohn, Natascha Katharina
Niedl, Robert Raimund
Anielski, Alexander
Lisdat, Fred
Beta, Carsten
BIOSENSORS-BASEL, 2022, 12 (02):
[42] Electrophoretic migration of DNA in lyotropic liquid crystals
Åkerman, B
Svingen, R
Carlsson, N
PHYSICAL CHEMISTRY OF INTERFACES AND NANOMATERIALS II, 2003, 5223 : 253 - 263
[43] Densitometric DNA Analysis in Agarose Electrophoretic Gels
Krizman, Mitja
Jakse, Jernej
Prosek, Mirko
Baricevic, Dea
Javornik, Branka
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC, 2009, 22 (03) : 167 - 170
[44] DNA Nanotechnology in the Undergraduate Laboratory: Electrophoretic Analysis of DNA Nanostructure Biostability
Chandrasekaran, Arun Richard
JOURNAL OF CHEMICAL EDUCATION, 2023, 100 (01) : 316 - 320
[45] Modification of the electrokinetic properties of reversible electrophoresis gels for the separation and preparation of DNA -: Addition of linear polymers
Cole, KD
Tellez, CM
Nguyen, RB
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1999, 82 (01) : 57 - 76
[46] Stepwise gradient of linear polymer matrices in microchip electrophoresis for high-resolution separation of DNA
Zhang, LH
Dang, FQ
Baba, Y
ELECTROPHORESIS, 2002, 23 (14) : 2341 - 2346
[47] Nonlinear electrophoretic response yields a unique parameter for separation of biomolecules
Pel, Joel
Broemeling, David
Mai, Laura
Poon, Hau-Ling
Tropini, Giorgia
Warren, Rene L.
Holt, Robert A.
Marziali, Andre
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (35) : 14796 - 14801
[48] Recent innovations in protein separation on microchips by electrophoretic methods: An update
Tran, Nguyet Thuy
Ayed, Ichraf
Pallandre, Antoine
Taverna, Myriam
ELECTROPHORESIS, 2010, 31 (01) : 147 - 173
[49] Modeling the Electrophoretic Separation of Short Biological Molecules in Nanofluidic Devices
Fayad, Ghassan N.
Hadjiconstantinou, Nicolas G.
JOURNAL OF FLUIDS ENGINEERING-TRANSACTIONS OF THE ASME, 2013, 135 (02):
[50] Role of Nanocomposite Hydrogel Morphology in the Electrophoretic Separation of Biomolecules: A Review
Simhadri, Jyothirmai J.
Stretz, Holly A.
Oyanader, Mario
Arce, Pedro E.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2010, 49 (23) : 11866 - 11877

← 1 2 3 4 5 →