Inhibitory effect of aqueous extract of Cuminum cyminum L. seed on degranulation of RBL-2H3 cells and passive cutaneous anaphylaxis reaction in mice

被引：11

作者：

Hada, Makoto ^{[1
]}

Nishi, Kosuke ^{[1
,2
]}

Ishida, Momoko ^{[1
]}

Onda, Hiroyuki ^{[3
]}

Nishimoto, Sogo ^{[4
]}

Sugahara, Takuya ^{[1
,2
]}

机构：

[1] Ehime Univ, Dept Biosci, Grad Sch Agr, Matsuyama, Ehime 7908566, Japan

[2] Ehime Univ, Food & Hlth Sci Res Ctr, Matsuyama, Ehime 7908566, Japan

[3] S&B Foods Inc, Cent Res Inst, Itabashi Ku, Tokyo 1748651, Japan

[4] Ishikawa Prefectural Univ, Dept Food Sci, Fac Bioresources & Environm Sci, Nonoichi, Ishikawa 9218836, Japan

来源：

CYTOTECHNOLOGY | 2019年 / 71卷 / 02期

关键词：

Anti-allergic effect; Cuminum cyminum L; Degranulation; beta-Hexosaminidase; Passive cutaneous anaphylaxis; RBL-2H3; cells; IGE; DISRUPTION;

D O I：

10.1007/s10616-019-00309-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cuminum cyminum L. (cumin) seed is used as a spice in various countries. Although several functions of the components in cumin seed have been reported, the anti-allergic effect of the water-soluble component in cumin seed has not been reported yet. In this study, we focused on the suppressive effect of cumin seed aqueous extract on degranulation in order to reveal the anti-allergic effect of cumin. Cumin seed aqueous extract significantly suppressed the antigen-induced degranulation of rat basophilic leukemia cell line RBL-2H3 cells in a dose-dependent manner without cytotoxicity. The extract also inhibited the elevation of the intracellular calcium ion concentration induced by antigen. Immunoblot analysis revealed that the extract suppresses phosphorylation of phosphatidylinositol 3-kinase, Bruton's tyrosine kinase, phospholipase C-gamma 1/2, and Akt in the signaling pathways activated by antigen induction via Fc epsilon RI. Furthermore, the extract suppressed microtubule formation induced by antigen. In addition, oral administration of cumin seed aqueous extract significantly suppressed the passive cutaneous anaphylaxis reaction in BALB/c mice. Our findings suggest that cumin seed contains water-soluble components with the anti-allergic effect. Therefore, cumin seed has potential as anti-allergic functional food.

引用

页码：599 / 609

页数：11

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