Protein dynamics from time resolved UV Raman spectroscopy

被引:61
|
作者
Balakrishnan, Gurusamy [1 ]
Weeks, Colin L. [1 ]
Ibrahim, Mohammed [1 ]
Soldatova, Alexandra V. [1 ]
Spiro, Thomas G. [1 ]
机构
[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1016/j.sbi.2008.06.001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Raman spectroscopy can provide unique information on the evolution of structure in proteins over a wide range of time scales; the picosecond to millisecond range can be accessed with pump-probe techniques. Specific parts of the molecule are interrogated by tuning the probe laser to a resonant electronic transition, including the UV transitions of aromatic residues and of the peptide bond. Advances in laser technology have enabled the characterization of transient species at an unprecedented level of structural detail. Applications to protein unfolding and allostery are reviewed.
引用
收藏
页码:623 / 629
页数:7
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