Preparation and Characterization of Tropolone-encapsulated Chitosan Nanoparticle for Application as Cosmetic Preservatives

As the life's level has been improved due to the development of industry, attention to the beauty has been increased. Due to this trend, many cosmetic industries developed and launched multi-functional cosmetics, but many of them are disposed due to the expiration date, deterioration and corruption of the products. Currently, tropolone preservative has been used as a method to prevent the degeneration or corruption of cosmetics, but its utilization is limited due to high cost and toxicity caused by overuse. To solve these problems, LMWSC-g-SA (LMSA) in this study was synthesized by using hydrophobic stearic acid (SA) modified in low molecular weight water-soluble chitosan (LMWSC) with coupling agent EDC, and cosmetic preservative with the sustainable drug release was developed through encapsulation of tropolone in LMSA. Physicochemical properties of LMSA were analyzed by H-1 NMR, DLS, fluorophotometer and TEM. In addition, encapsulation efficiency and release behavior of tropolone were confirmed by UV-vis spectrophotometer. Moreover, cytotoxicity of LMSA was evaluated by MTT assay. Through these results, we suggest that LMSAT is very superb substance because this can induce the maximum effect of tropolone when was used to cosmetic preservative.