Upregulation of B23 promotes tumor cell proliferation and predicts poor prognosis in glioma

被引：10

作者：

Chen, Jianguo ^{[1
,2
]}

Sun, Jie ^{[2
]}

Yang, Liu ^{[2
]}

Yan, Yaohua ^{[2
]}

Shi, Wei ^{[2
]}

Shi, Jinlong ^{[2
]}

Huang, Qingfeng ^{[2
]}

Chen, Jian ^{[2
]}

Lan, Qing ^{[1
]}

机构：

[1] Soochow Univ, Affiliated Hosp 2, Dept Neurosurg, Suzhou 215004, Jiangsu, Peoples R China

[2] Nantong Univ, Affiliated Hosp, Dept Neurosurg, Nantong 226001, Jiangsu, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2015年 / 466卷 / 01期

关键词：

B23; Glioma; Prognosis; Proliferation; MALIGNANT GLIOMAS; TRANSCRIPTIONAL ACTIVITY; SUPPRESSOR PROTEIN; NUCLEOPHOSMIN/B23; EXPRESSION; THERAPY; CANCER; CENTROSOME; CARCINOMA; DIAGNOSIS;

D O I：

10.1016/j.bbrc.2015.08.118

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

B23 (also known as Nucleophosmin, NPM, numatrin or NO38) is a ubiquitously expressed phosphoprotein belonging to the nucleoplasmin family of chaperones. In this study we intended to investigate the clinical significance of B23 expression in human glioma and its biological function in glioma cells. Western blot and immunohistochemistry analysis showed that B23 was overexpressed in glioma tissues and glioma cell lines. In addition, the expression level of B23 was positively correlated with glioma pathological grade and Ki-67 expression. Kaplan-Meier analysis revealed that a higher B23 expression in patients with glioma was associated with a poorer prognosis. In vitro, after the release of glioma cell lines from serum starvation, the expression of B23 was upregulated, as well as PCNA (Proliferating Cell Nuclear Antigen) and cyclin A. In addition, knockdown of B23 by small interfering RNA transfection diminished the expression of PCNA, cyclin D1 and arrested cell growth at G1 phase. Taken together, our results implied that B23 could be a candidate prognostic biomarker as well as a potential therapeutical target of glioma. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：124 / 130

页数：7

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