Three-Dimensional Paper-Based Microfluidic Analysis Device for Simultaneous Detection of Multiple Biomarkers with a Smartphone

被引:39
作者
Baek, Seung Ho [1 ]
Park, Chanyong [2 ]
Jeon, Jaehyung [1 ]
Park, Sungsu [1 ,2 ,3 ]
机构
[1] Sungkyunkwan Univ, Sch Mech Engn, Suwon 16419, South Korea
[2] Sungkyunkwan Univ, Dept Biomed Engn, Suwon 16419, South Korea
[3] Sungkyunkwan Univ, Biomed Inst Convergence SKKU BICS, Suwon 16419, South Korea
来源
BIOSENSORS-BASEL | 2020年 / 10卷 / 11期
基金
新加坡国家研究基金会;
关键词
paper-based microfluidic device; colorimetric; multiple detection; smartphone application; ALKALINE-PHOSPHATASE; GLUCOSE; INTENSITY; DISEASE; ASSAY;
D O I
10.3390/bios10110187
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Paper-based microfluidic analysis devices (mu PADs) have attracted attention as a cost-effective platform for point-of-care testing (POCT), food safety, and environmental monitoring. Recently, three-dimensional (3D)-mu PADs have been developed to improve the performance of mu PADs. For accurate diagnosis of diseases, however, 3D-mu PADs need to be developed to simultaneously detect multiple biomarkers. Here, we report a 3D-mu PADs platform for the detection of multiple biomarkers that can be analyzed and diagnosed with a smartphone. The 3D-mu PADs were fabricated using a 3D digital light processing printer and consisted of a sample reservoir (300 mu L) connected to 24 detection zones (of 4 mm in diameter) through eight microchannels (of 2 mm in width). With the smartphone application, eight different biomarkers related to various diseases were detectable in concentrations ranging from normal to abnormal conditions: glucose (0-20 mmol/L), cholesterol (0-10 mmol/L), albumin (0-7 g/dL), alkaline phosphatase (0-800 U/L), creatinine (0-500 mu mol/L), aspartate aminotransferase (0-800 U/L), alanine aminotransferase (0-1000 U/L), and urea nitrogen (0-7.2 mmol/L). These results suggest that 3D-mu PADs can be used as a POCT platform for simultaneous detection of multiple biomarkers.
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页数:12
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