PFTK1 promotes the progression of non-small cell lung cancer (NSCLC) through the Wnt/β-catenin signaling pathway

被引：0
作者：
Jia, Liang-Liang [1 ,2 ]
Chen, Jie [3 ]
Gai, Ling [4 ]
Li, Yu-Peng [5 ]
Lu, Xiao-Ning [6 ]
Cui, Xue-Fan [1 ]
Ji, Li-Li [2 ,7 ]
机构：
[1] Nanjing Med Univ, Affiliated Hosp 1, Dept Resp Med, Nanjing, Jiangsu, Peoples R China
[2] Nantong Univ, Dept Pathol, Coll Med, Nantong, Jiangsu, Peoples R China
[3] Jiangyin Peoples Hosp, Dept Oncol, Jiangyin, Jiangsu, Peoples R China
[4] Nantong Univ, Affiliated Hosp, Dept Oncol, Nantong, Jiangsu, Peoples R China
[5] Peoples Hosp Rizhao City, Dept Pediat, Rizhao, Shandong, Peoples R China
[6] Nantong Univ, Affiliated Hosp, Dept Thorac Surg, Nantong, Jiangsu, Peoples R China
[7] Nantong Univ, Coll Med, Dept Jiangsu Prov, Key Lab Inflammat & Mol Drug Target, Nantong, Jiangsu, Peoples R China
来源：
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY | 2016年 / 9卷 / 12期
基金：
中国国家自然科学基金;
关键词：
PFTK1; NSCLC; proliferation; Wnt; BETA-CATENIN; CYCLIN Y; HEPATOCELLULAR-CARCINOMA; NEGATIVE REGULATOR; WNT; IDENTIFICATION; KINASE; DISEASE; TARGET;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
PFTK1, as a Cdc-related serine/threonine protein kinase, is a crucial regulator of cyclins and cell cycle. However, the role of PFTK1 in non-small cell lung cancer (NSCLC) remains elusive. In the present study, Western blot and immunohistochemistry (IHC) assays demonstrated that PFTK1 was up-regulated in lung cancer tissues and cell lines. In addition, IHC analysis revealed that PFTK1 expression was associated with histological differentiation, lymph node status, TNM stage and predicted poor prognosis of NSCLC. In vitro studies using serum starvation-refeeding experiment and PFTK1 overexpression assay demonstrated that PFTK1 expression promoted proliferation of NSCLC cells, while PFTK1 knockdown assay showed that PFTK1 knockdown led to decreased cell growth rate. Besides, the transcription activity of Wnt/beta-catenin pathway was enhanced when PFTK1 was overexpressed. Our finding supported that up-regulated PFTK1 might promote the progression of NSCLC via activating the Wnt/beta-catenin signaling pathway.
引用
收藏
页码：12228 / 12239
页数：12
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