Potential of Centrifugal Seeding Method in Improving Cells Distribution and Proliferation on Demineralized Cancellous Bone Scaffolds for Tissue-Engineered Meniscus

被引:27
作者
Zhang, Zheng-Zheng [1 ]
Jiang, Dong [1 ]
Wang, Shao-Jie [1 ]
Qi, Yan-Song [1 ]
Zhang, Ji-Ying [1 ]
Yu, Jia-Kuo [1 ]
机构
[1] Peking Univ, Hosp 3, Beijing Key Lab Sports Injuries, Inst Sports Med, Beijing 100191, Peoples R China
基金
国家高技术研究发展计划(863计划);
关键词
tissue-engineered meniscus; seeding method; meniscal fibrochondrocytes; mesenchymal stem cells; demineralized cancellous bone; MESENCHYMAL STEM-CELLS; POLYMER SCAFFOLDS; IN-VIVO; REPAIR; BIOMATERIALS; DEFECTS; CULTURE; MARROW; FORCE;
D O I
10.1021/acsami.5b03129
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Tissue-engineered meniscus offers a possible solution to the regeneration and replacement problem of meniscectomy. However, the nonuniform distribution and declined proliferation of seeded cells on scaffolds hinder the application of tissue-engineered meniscus as a new generation of meniscus graft. This study systematically investigated the performances of different seeding techniques by using the demineralized cancellous bone (DCB) as the scaffold. Static seeding, injection seeding, centrifugal seeding, and vacuum seeding methods were used to seed the meniscal fibrochondrocytes (MFCs) and mesenchymal stem cells (MSCs) to scaffolds. Cell-binding efficiency, survival rate, distribution ability, and long-term proliferation effects on scaffolds were quantitatively evaluated. Cell adhesion was compared via cell-binding kinetics. Cell viability and morphology were assessed by using fluorescence staining. Combined with the reconstructed three-dimensional image, the distribution of seeded cells was investigated. The Cell Counting Kit-8 assay and DNA assay were employed to assess cell proliferation. Cell-binding kinetics and cell survival of the MFCs were improved via centrifugal seeding compared to injection or vacuum seeding methods. Seeded MFCs by centrifugation showed a more homogeneous distribution throughout the scaffold than cells seeded by other methods. Moreover, the penetration depth in the scaffold of seeded MFCs by centrifugation was 300-500 mu m, much higher than the value of 100-300 mu m by the surface static and injection seeding. The long-term proliferation of the MFCs in the centrifugal group was also significantly higher than that in the other groups. The results of the MSCs were similar to those of the MFCs. The centrifugal seeding method could significantly improve MFCs or MSCs distribution and proliferation on the DCB scaffolds, thus providing a simple, cost-effective, and effective cell-seeding protocol for tissue-engineered meniscus.
引用
收藏
页码:15294 / 15302
页数:9
相关论文
共 32 条
  • [1] The effect of nanofiber alignment on the maturation of engineered meniscus constructs
    Baker, Brendon M.
    Mauck, Robert L.
    [J]. BIOMATERIALS, 2007, 28 (11) : 1967 - 1977
  • [2] Tissue engineering of the meniscus
    Buma, P
    Ramrattan, NN
    van Tienen, TG
    Veth, RPH
    [J]. BIOMATERIALS, 2004, 25 (09) : 1523 - 1532
  • [3] Adhesion of mesenchymal stem cells to polymer scaffolds occurs via distinct ECM ligands and controls their osteogenic differentiation
    Chastain, Sara R.
    Kundu, Anup K.
    Dhar, Sanjay
    Calvert, Jay W.
    Putnam, Andrew J.
    [J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2006, 78A (01) : 73 - 85
  • [4] Long-term outcome for large meniscal defects treated with small intestinal submucosa in a dog model
    Cook, JL
    Fox, DB
    Malaviya, P
    Tomlinson, JL
    Kuroki, K
    Cook, CR
    Kladakis, S
    [J]. AMERICAN JOURNAL OF SPORTS MEDICINE, 2006, 34 (01) : 32 - 42
  • [5] One-Step Repair for Cartilage Defects in a Rabbit Model A Technique Combining the Perforated Decalcified Cortical-Cancellous Bone Matrix Scaffold With Microfracture
    Dai, Linghui
    He, Zhenming
    Zhang, Xin
    Hu, Xiaoqing
    Yuan, Lan
    Qiang, Ming
    Zhu, Jingxian
    Shao, Zhenxing
    Zhou, Chunyan
    Ao, Yingfang
    [J]. AMERICAN JOURNAL OF SPORTS MEDICINE, 2014, 42 (03) : 583 - 591
  • [6] Dai WD, 2009, BIOSCI TRENDS, V3, P216
  • [7] Study of biomedical specimens using scanning acoustic microscopy
    Doroski, D.
    Tittmann, B. R.
    Miyasaka, C.
    [J]. ACOUSTICAL IMAGING, VOL 28, 2007, 28 : 13 - +
  • [8] A New Source of Mesenchymal Stem Cells for Articular Cartilage Repair MSCs Derived From Mobilized Peripheral Blood Share Similar Biological Characteristics In Vitro and Chondrogenesis In Vivo as MSCs From Bone Marrow in a Rabbit Model
    Fu, Wei-Li
    Zhou, Chun-Yan
    Yu, Jia-Kuo
    [J]. AMERICAN JOURNAL OF SPORTS MEDICINE, 2014, 42 (03) : 592 - 601
  • [9] Goble EM, 1999, SCAND J MED SCI SPOR, V9, P146
  • [10] A novel use of centrifugal force for cell seeding into porous scaffolds
    Godbey, WT
    Hindy, BSS
    Sherman, ME
    Atala, A
    [J]. BIOMATERIALS, 2004, 25 (14) : 2799 - 2805