Indication of metabolic inflexibility to food intake in spontaneously overweight Labrador Retriever dogs

被引:11
作者
Soder, Josefin [1 ]
Wernersson, Sara [1 ]
Dicksved, Johan [2 ]
Hagman, Ragnvi [3 ]
Ostman, Johnny R. [4 ]
Moazzami, Ali A. [4 ]
Hoglund, Katja [1 ]
机构
[1] Swedish Univ Agr Sci, Fac Vet Med & Anim Sci, Dept Anat Physiol & Biochem, Box 7011, S-75007 Uppsala, Sweden
[2] Swedish Univ Agr Sci, Fac Vet Med & Anim Sci, Dept Anim Nutr & Management, Box 7024, S-75007 Uppsala, Sweden
[3] Swedish Univ Agr Sci, Fac Vet Med & Anim Sci, Dept Clin Sci, Box 7054, S-75007 Uppsala, Sweden
[4] Swedish Univ Agr Sci, Fac Nat Resources & Agr Sci, Dept Mol Sci, Box 7015, S-75007 Uppsala, Sweden
基金
瑞典研究理事会;
关键词
Acetylcarnitine; Canine; Carnitine; Fatty-acid oxidation; Feed-challenge test; LC-TOFMS; Lipidomics; Liquid chromatography-time of flight mass spectrometry; Obesity; Phospholipids; SKELETAL-MUSCLE; OBESE DOGS; INSULIN-RESISTANCE; COMPANION ANIMALS; ACID; GLUCOSE; WEIGHT; CANINE; DYSFUNCTION; ACYLCARNITINES;
D O I
10.1186/s12917-019-1845-5
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
BackgroundObesity in dogs is an increasing problem associated with morbidity, shortened life span and poor life quality. Overweight dogs exhibit postprandial hyperlipidaemia, highlighting the need to identify potential dysregulations in lipid metabolism. This study investigated metabolites related to lipid metabolism (i.e. acylcarnitines and taurine) and phospholipids in a feed-challenge test and aimed to identify metabolic variations in spontaneously overweight dogs. Twenty-eight healthy male Labrador Retriever dogs were included, 12 of which were classified as lean (body condition score (BCS) 4-5 on a 9-point scale) and 16 as overweight (BCS 6-8). After overnight fasting (14-17h), fasting blood samples were collected and dogs were fed a high-fat meal followed by postprandial blood sample collection hourly for 4h. Liquid chromatography-time of flight mass spectrometry (LC-TOFMS) was used to identify plasma metabolites and phospholipids. Multivariate models, mixed model repeated measures and linear regression analyses were used for data interpretation.ResultsIn all dogs, propionylcarnitine, stearoylcarnitine and nine phospholipids increased in response to food intake, while vaccenylcarnitine decreased (P0.005 for all). Overall, carnitine and acetylcarnitine signal areas in the feed-challenge test were lower in overweight dogs (P0.004). Notably, fasting plasma acetylcarnitine was lower in overweight dogs than in lean dogs (P=0.001) and it did not change in response to feeding. The latter finding was in contrast to the decreased acetylcarnitine signal area found in lean dogs at one hour postprandially (P<0.0001). One fasting phosphatidylcholine (PCaa C38:4) was higher in prominently overweight dogs (BCS>6) than in lean dogs (P<0.05).ConclusionsPlasma carnitine status was overall lower in spontaneously overweight dogs than in lean dogs in this cohort of healthy Labrador Retriever dogs, indicating a potential carnitine insufficiency in the overweight group. The acetylcarnitine response in overweight dogs indicated decreased fatty acid oxidation at fasting and metabolic inflexibility to food intake. Further studies on metabolic inflexibility and its potential role in the metabolism of overweight dogs are warranted.
引用
收藏
页数:11
相关论文
共 68 条
[1]   Evidence of longer life; a cohort of 39 labrador retrievers [J].
Adams, Vicki J. ;
Ceccarelli, Kellie ;
Watson, Penny ;
Carmichael, Stuart ;
Penell, Johanna ;
Morgan, David M. .
VETERINARY RECORD, 2018, 182 (14) :408
[2]   Short-term obesity results in detrimental metabolic and cardiovascular changes that may not be reversed with weight loss in an obese dog model [J].
Adolphe, Jennifer L. ;
Silver, Tawni I. ;
Childs, Helene ;
Drew, Murray D. ;
Weber, Lynn P. .
BRITISH JOURNAL OF NUTRITION, 2014, 112 (04) :647-656
[3]   Health Effects of Overweight and Obesity in 195 Countries over 25 Years [J].
Afshin, Ashkan ;
Forouzanfar, Mohammad H. ;
Reitsma, Marissa B. ;
Sur, Patrick ;
Estep, Kara ;
Lee, Alex ;
Marczak, Laurie ;
Mokdad, Ali H. ;
Moradi-Lakeh, Maziar ;
Naghavi, Mohsen ;
Salama, Joseph S. ;
Vos, Theo ;
Abate, Kalkidan H. ;
Abbafati, Cristiana ;
Ahmed, Muktar B. ;
Al-Aly, Ziyad ;
Alkerwi, Ala'a ;
Al-Raddadi, Rajaa ;
Amare, Azmeraw T. ;
Amberbir, Alemayehu ;
Amegah, Adeladza K. ;
Amini, Erfan ;
Amrock, Stephen M. ;
Anjana, Ranjit M. ;
Arnlov, Johan ;
Asayesh, Hamid ;
Banerjee, Amitava ;
Barac, Aleksandra ;
Baye, Estifanos ;
Bennett, Derrick A. ;
Beyene, Addisu S. ;
Biadgilign, Sibhatu ;
Biryukov, Stan ;
Bjertness, Espen ;
Boneya, Dube J. ;
Campos-Nonato, Ismael ;
Carrero, Juan J. ;
Cecilio, Pedro ;
Cercy, Kelly ;
Ciobanu, Liliana G. ;
Cornaby, Leslie ;
Damtew, Solomon A. ;
Dandona, Lalit ;
Dandona, Rakhi ;
Dharmaratne, Samath D. ;
Duncan, Bruce B. ;
Eshrati, Babak ;
Esteghamati, Alireza ;
Feigin, Valery L. ;
Fernandes, Joao C. .
NEW ENGLAND JOURNAL OF MEDICINE, 2017, 377 (01) :13-27
[4]   Associations of anthropometric markers with serum metabolites using a targeted metabolomics approach: results of the EPIC-potsdam study [J].
Bachlechner, U. ;
Floegel, A. ;
Steffen, A. ;
Prehn, C. ;
Adamski, J. ;
Pischon, T. ;
Boeing, H. .
NUTRITION & DIABETES, 2016, 6 :e215-e215
[5]   Lipoproteins abnormalities in obese insulin-resistant dogs [J].
Bailhache, E ;
Nguyen, P ;
Krempf, M ;
Siliart, B ;
Magot, T ;
Ouguerram, K .
METABOLISM-CLINICAL AND EXPERIMENTAL, 2003, 52 (05) :559-564
[6]   Metabolomic Analysis Reveals Altered Skeletal Muscle Amino Acid and Fatty Acid Handling in Obese Humans [J].
Baker, Peter R., II ;
Boyle, Kristen E. ;
Koves, Timothy R. ;
Ilkayeva, Olga R. ;
Muoio, Deborah M. ;
Houmard, Joseph A. ;
Friedman, Jacob E. .
OBESITY, 2015, 23 (05) :981-988
[7]   Physical inactivity as the culprit of metabolic inflexibility: evidence from bed-rest studies [J].
Bergouignan, Audrey ;
Rudwill, Floriane ;
Simon, Chantal ;
Blanc, Stephane .
JOURNAL OF APPLIED PHYSIOLOGY, 2011, 111 (04) :1201-1210
[8]   Alterations of plasma metabolite profiles related to adipose tissue distribution and cardiometabolic risk [J].
Boulet, Marie Michele ;
Chevrier, Genevieve ;
Grenier-Larouche, Thomas ;
Pelletier, Melissa ;
Nadeau, Melanie ;
Scarpa, Julia ;
Prehn, Cornelia ;
Marette, Andre ;
Adamski, Jerzy ;
Tchernof, Andre .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2015, 309 (08) :E736-E746
[9]   Obesity and Associated Comorbidities in People and Companion Animals: A One Health Perspective [J].
Chandler, M. ;
Cunningham, S. ;
Lund, E. M. ;
Khanna, C. ;
Naramore, R. ;
Patel, A. ;
Day, M. J. .
JOURNAL OF COMPARATIVE PATHOLOGY, 2017, 156 (04) :296-309
[10]  
Cole L, 2016, TXB HUMAN PHYSL BIOC