Reorganisation of a mesophilic biogas microbiome as response to a stepwise increase of ammonium nitrogen induced by poultry manure supply

被引：35

作者：

Alsouleman, Khulud ^{[1
,2
]}

Linke, Bernd ^{[1
]}

Klang, Johanna ^{[1
]}

Klocke, Michael ^{[1
]}

Krakat, Niclas ^{[1
]}

Theuerl, Susanne ^{[1
]}

机构：

[1] Leibniz Inst Agr Engn Potsdam Bornim ATB, Dept Bioengn, Max Eyth Allee 100, D-14469 Potsdam, Germany

[2] Univ Gottingen, Fac Agr Sci, Busgenweg 5, D-37077 Gottingen, Germany

来源：

BIORESOURCE TECHNOLOGY | 2016年 / 208卷

关键词：

Anaerobic digestion; Poultry manure; Disturbance; Microbial community reconstruction; NH4+-N inhibition; ANAEROBIC-DIGESTION PROCESS; COMMUNITY STRUCTURE; TEMPERATURE; INHIBITION; BACTERIAL; METHANE;

D O I：

10.1016/j.biortech.2016.02.104

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

An anaerobic digestion experiment was investigated to evaluate the impact of increasing amounts of ammonium nitrogen due to poultry manure addition on the reactor performance, especially on the microbiome response. The microbial community structure was assessed by using a 16S rRNA gene approach, which was further correlated with the prevalent environmental conditions by using statistical analyses. The addition of 50% poultry manure led to a process disturbance indicated by a high VFA content (almost 10 g(HAc-Eq) L (1)) in combination with elevated concentrations of ammonium nitrogen (5.9 g NH4+-N kg(FM)(1)) and free ammonia (0.5 g NH3 kg(FM)(1)). Simultaneously the microbiome, changed from a Bacteroidetes-dominated to a Clostridiales-dominated community accompanied by a shift from the acetoclastic to the hydrogenotrophic pathway. The "new" microbial community was functional redundant as the overall process rates were similar to the former one. A further increase of poultry manure resulted in a complete process failure. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：200 / 204

页数：5

共 19 条

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