Dual-channel red fluorescent probe for detection of Cys/Hcy and GSH in plants

被引：61

作者：

Huang, Yongfei ^{[1
]}

Zhang, Yongbin ^{[2
]}

Huo, Fan Gjun ^{[2
]}

Liu, Yaoming ^{[3
]}

Yin, Caixia ^{[1
]}

机构：

[1] Shanxi Univ, Key Lab Chem Biol & Mol Engn, Key Lab Mat Energy Convers & Storage Shanxi Prov, Minist Educ,Inst Mol Sci, Taiyuan 030006, Shanxi, Peoples R China

[2] Shanxi Univ, Res Inst Appl Chem, Taiyuan 030006, Shanxi, Peoples R China

[3] Shanxi Univ, Sci Instrument Ctr, Taiyuan, Shanxi, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2019年 / 301卷

基金：

山西省青年科学基金; 中国国家自然科学基金; 山西省归国人员基金;

关键词：

Biothiols; Discrimination detection; Red fluorescent probe; Imaging; Plants; HIGHLY SELECTIVE DETECTION; CYSTEINE; HOMOCYSTEINE; GLUTATHIONE; CYS; DISCRIMINATION; LUMINESCENT; CONJUGATE; BIOTHIOLS; BODIPY;

D O I：

10.1016/j.snb.2019.127123

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Cysteine (Cys), Glutathione (GSH) can resist the reactive oxygen species due to adversity and aging in plants. Thus, the specific detection of individual thiol in plants is very important. However, just a few have been used to discrimination of Cys/Hcy and GSH in vivo. Not to mention the discrimination detection for them in plants. In this work, we synthesized a potential promoting tandem reaction type fluorescent probe based on benzene-conjugated benzopylium ion and NBD-Cl. The probe could detect Cys/Hcy and GSH with high selectivity and low detection limit. More interesting, firstly we employed the probe to successfully detect Cys/Hcy and GSH in arabidopsis thaliana. Our research is useful for studying the conversion and metabolic pathways of thiols in plants.

引用

页数：8

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