The Combination of Scutellaria baicalensis Georgi and Sophora japonica L. ameliorate Renal Function by Regulating Gut Microbiota in Spontaneously Hypertensive Rats

被引:23
作者
Guan, Yiqing [1 ]
Chen, Kai [1 ]
Quan, Dongling [2 ]
Kang, Liangqi [1 ]
Yang, Danni [2 ]
Wu, Huanxian [2 ]
Yan, Mengqiu [2 ]
Wu, Shaoyu [2 ]
Lv, Lin [2 ]
Zhang, Guohua [1 ]
机构
[1] Southern Med Univ, Sch Tradit Chinese Med, Guangzhou, Peoples R China
[2] Southern Med Univ, Sch Pharmaceut Sci, Guangzhou, Peoples R China
来源
FRONTIERS IN PHARMACOLOGY | 2021年 / 11卷
关键词
Scutellaria baicalensis Georgi; Sophora japonica L; hypertensive nephropathy; gut microbiota; traditional Chinese medicine;
D O I
10.3389/fphar.2020.575294
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Chronic kidney disease (CKD) is becoming a notable health concern globally. The combination of Scutellaria baicalensis Georgi (SB) and Sophora japonica L. (SJ) has been demonstrated to have anti-hypertensive effects and improve kidney injury clinically. This study aimed to explore the renal protective effect of the combination of SB and SJ against CKD and clarify the potential mechanisms. Male spontaneously hypertensive rats (SHR) were used to induce hypertensive nephropathy and were treated with SB or SJ separately or in combination for 15 weeks, and an antibiotic group was used for a rescue experiment. Blood pressure, serum or urine biochemical markers, serum inflammation factors, short-chain fatty acids (SCFAs), indoxyl sulfate (IS), and oxidative stress indicators were assessed. Western blot analysis was performed to determine the expression of intestinal tight junction proteins, including occludin and ZO-1. The mRNA expression of the SCFAs receptors olfactory 78 (Olfr78) and G protein-coupled receptor 41 (GPR41) was determined by quantitative real-time PCR. Gut microbiota profiles were established via high-throughput sequencing of the V3-V4 region of the bacterial 16S rRNA gene. SB and SJ significantly ameliorated the severity of renal injury induced by hypertension. The combination also decreased the ratio of Firmicutes/Bacteroidetes, increased the relative abundance of Lactobacillus, and reduced that of Clostridiaceae. The intestinal barrier was improved, and the change in dominant bacteria reduced IS accumulation and further inhibited oxidative stress activation in kidneys. SB and SJ increased SCFAs production, inhibited inflammatory factor release, and regulated blood pressure by decreasing the expression of Olfr78 and increasing that of GPR41, then alleviated kidney damage. This research demonstrated the positive effects of SB and SJ in a rat model of hypertensive nephropathy, indicated that the treatment of SB and SJ by improving the intestinal barrier function, increasing SCFAs, reducing inflammation, decreasing IS, and inhibiting oxidative stress reactions.
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页数:23
相关论文
共 36 条
  • [1] Update on Inflammation in Chronic Kidney Disease
    Akchurin, Oleh M.
    Kaskel, Frederick
    [J]. BLOOD PURIFICATION, 2015, 39 (1-3) : 84 - 92
  • [2] Assessment of Global Kidney Health Care Status
    Bello, Aminu K.
    Levin, Adeera
    Tonelli, Marcello
    Okpechi, Ikechi G.
    Feehally, John
    Harris, David
    Jindal, Kailash
    Salako, Babatunde L.
    Rateb, Ahmed
    Osman, Mohamed A.
    Qarni, Bilal
    Saad, Syed
    Lunney, Meaghan
    Wiebe, Natasha
    Johnson, David W.
    [J]. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2017, 317 (18): : 1864 - 1881
  • [3] Byung H.H., 1996, INT COLLATION TRADIT, P68
  • [4] Next-Generation Beneficial Microbes: The Case of Akkermansia muciniphila
    Cani, Patrice D.
    de Vos, Willem M.
    [J]. FRONTIERS IN MICROBIOLOGY, 2017, 8
  • [5] Developmental signalling pathways in renal fibrosis: the roles of Notch, Wnt and Hedgehog
    Edeling, Maria
    Ragi, Grace
    Huang, Shizheng
    Pavenstaedt, Hermann
    Susztak, Katalin
    [J]. NATURE REVIEWS NEPHROLOGY, 2016, 12 (07) : 426 - 439
  • [6] SELECTIVITY OF RENAL INJURY AND PROTEINURIA IN SPONTANEOUSLY HYPERTENSIVE RAT
    FELD, LG
    VANLIEW, JB
    GALASKE, RG
    BOYLAN, JW
    [J]. KIDNEY INTERNATIONAL, 1977, 12 (05) : 332 - 343
  • [7] Traditional Chinese Medicine for Cardiovascular Disease Evidence and Potential Mechanisms
    Hao, Panpan
    Jiang, Fan
    Cheng, Jing
    Ma, Lianyue
    Zhang, Yun
    Zhao, Yuxia
    [J]. JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2017, 69 (24) : 2952 - 2966
  • [8] Intestinal Dysbiosis Associated with Systemic Lupus Erythematosus
    Hevia, Arancha
    Milani, Christian
    Lopez, Patricia
    Cuervo, Adriana
    Arboleya, Silvia
    Duranti, Sabrina
    Turroni, Francesca
    Gonzalez, Sonia
    Suarez, Ana
    Gueimonde, Miguel
    Ventura, Marco
    Sanchez, Borja
    Margolles, Abelardo
    [J]. MBIO, 2014, 5 (05):
  • [9] Development of structural kidney damage in spontaneously hypertensive rats
    Hultstrom, Michael
    [J]. JOURNAL OF HYPERTENSION, 2012, 30 (06) : 1087 - 1091
  • [10] ISHIDA H, 1989, CHEM PHARM BULL, V37, P1616, DOI 10.1248/cpb.37.1616